Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100193, People's Republic of China.
College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, 071002, China.
Sci Rep. 2019 Aug 14;9(1):11835. doi: 10.1038/s41598-019-48365-0.
Ten new cucurbitane triterpenoids, hemsleyacins A-J (1-10), together with three known cucurbitane triterpenoids, dihydrocucurbitacin F (11), scandenogenin D (12), and jinfushanencin F (13), were separated from ethanolic tuber extracts of Hemsleya penxianensis. The absolute configurations of the new compounds were established based on NMR, HRESIMS, and CD spectra. Compounds 7 and 10-12 were evaluated in terms of their antifeedant activity against Plutella xylostella larvae. The result showed that compound 10 exhibited potent antifeedant activity against P. xylostella larvae after 48 h of treatment. Furthermore, the MTT test showed that compound 11 exhibited potent inhibition toward the UMUC-3 and T24 cell lines with IC values of 29.12 and 35.62 μM, respectively, compared to the positive control cisplatin IC values of 8.27 and 13.72 μM. Western blot analysis revealed that compound 11 treatments substantially inhibited the phosphorylation of IκBα.
从滇南雪胆（Hemsleya penxianensis）的乙醇根提取部分中分离得到了 10 个新的葫芦烷三萜化合物，分别命名为 hemsleyacins A-J（1-10），以及 3 个已知的葫芦烷三萜化合物，分别是二氢葫芦素 F（11）、 scandogenin D（12）和金佛山恩慈宁 F（13）。根据 NMR、HRESIMS 和 CD 光谱，确定了新化合物的绝对构型。评估了化合物 7 和 10-12 对小菜蛾幼虫的拒食活性。结果表明，化合物 10 在处理 48 小时后对小菜蛾幼虫表现出很强的拒食活性。此外，MTT 试验显示，与阳性对照顺铂的 IC 值（8.27 和 13.72 μM）相比，化合物 11 对 UMUC-3 和 T24 细胞系的抑制活性较强，IC 值分别为 29.12 和 35.62 μM。Western blot 分析显示，化合物 11 处理可显著抑制 IκBα 的磷酸化。
