Bai Jie, Xu Jian, Zhao Jian, Zhang Rui
Department of Breast Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shengyang, Liaoning 110042, P.R. China.
Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shengyang, Liaoning 110042, P.R. China.
Oncol Lett. 2019 Aug;18(2):2007-2012. doi: 10.3892/ol.2019.10515. Epub 2019 Jun 21.
Long non-coding RNA (lncRNA) associated with poor prognosis of hepatocellular carcinoma (AWPPH) serves pivotal roles in bladder cancer and liver cancer; however, to the best of our knowledge, its functionality in colon cancer has not been characterized. The present study aimed to investigate the involvement of lncRNA AWPPH in colon cancer. Serum levels of lncRNA AWPPH and glucose transporter 1 (GLUT-1) in patients with early stage colon cancer and healthy controls were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and ELISA. Correlation between lncRNA AWPPH and GLUT-1 expression was analyzed by Pearson's correlation coefficient. χ test was performed to investigate the associations between serum levels of lncRNA AWPPH and clinical data of patients with colon cancer. lncRNA AWPPH short hairpin RNA and GLUT-1 expression vectors were transfected into colon cancer cells, and the effects on lncRNA AWPPH, GLUT-1 and cell proliferation were detected by RT-qPCR, western blotting and Cell Counting Kit-8 assay. It was observed that serum levels of lncRNA AWPPH and GLUT-1 were significantly higher in patients with colon cancer patients compared with healthy controls. Serum levels of AWPPH and GLUT-1 were significantly positively correlated in patients with colon cancer. Serum levels of lncRNA AWPPH were associated with the tumor size. Furthermore, AWPPH-silencing significantly inhibited GLUT-1 expression and inhibited cancer cell proliferation. GLUT-1 overexpression promoted cancer cell proliferation and attenuated the inhibitory effects of AWPPH-silencing on cancer cell proliferation. However, GLUT-1 overexpression failed to significantly affect the expression of AWPPH. Therefore, it can be concluded that a downregulation of lncRNA AWPPH may inhibit colon cancer cell proliferation by downregulating GLUT-1.
与肝细胞癌预后不良相关的长链非编码RNA（lncRNA）（AWPPH）在膀胱癌和肝癌中起关键作用；然而，据我们所知，其在结肠癌中的功能尚未得到阐明。本研究旨在探讨lncRNA AWPPH在结肠癌中的作用。通过逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）检测早期结肠癌患者和健康对照者血清中lncRNA AWPPH和葡萄糖转运蛋白1（GLUT-1）的水平。采用Pearson相关系数分析lncRNA AWPPH与GLUT-1表达之间的相关性。进行χ检验以研究lncRNA AWPPH血清水平与结肠癌患者临床数据之间的关联。将lncRNA AWPPH短发夹RNA和GLUT-1表达载体转染至结肠癌细胞中，通过RT-qPCR、蛋白质印迹法和细胞计数试剂盒-8检测法检测其对lncRNA AWPPH、GLUT-1和细胞增殖的影响。结果观察到，与健康对照者相比，结肠癌患者血清中lncRNA AWPPH和GLUT-1水平显著更高。结肠癌患者血清中AWPPH和GLUT-1水平呈显著正相关。lncRNA AWPPH血清水平与肿瘤大小相关。此外，AWPPH沉默显著抑制GLUT-1表达并抑制癌细胞增殖。GLUT-1过表达促进癌细胞增殖并减弱AWPPH沉默对癌细胞增殖的抑制作用。然而，GLUT-1过表达未能显著影响AWPPH的表达。因此，可以得出结论，lncRNA AWPPH的下调可能通过下调GLUT-1来抑制结肠癌细胞增殖。
