一种用于检测囊性纤维化患者血清中抗铜绿假单胞菌IgA抗体的酶联免疫吸附测定法。
An ELISA to detect antipseudomonal IgA antibodies in sera of patients with cystic fibrosis.
作者信息
Brett M M, Ghoneim A T, Littlewood J M
机构信息
Department of Paediatrics, St James's University Hospital, Leeds.
出版信息
J Clin Pathol. 1988 Oct;41(10):1130-4. doi: 10.1136/jcp.41.10.1130.
Abstract
An enzyme linked immunosorbent assay (ELISA) to measure free serum IgA antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis is described. Results were reproducible and there was no interference from crossreacting antibodies directed against other Gram negative bacteria. Titres were high in patients with Pseudomonas aeruginosa infection and differed according to the stage of the infection. These preliminary results suggest that this assay may be of value in assessing the state of Pseudomonas aeruginosa infection in patients with cystic fibrosis.
摘要
本文描述了一种用于测量囊性纤维化患者血清中抗铜绿假单胞菌游离IgA抗体的酶联免疫吸附测定（ELISA）。结果具有可重复性，且不存在针对其他革兰氏阴性菌的交叉反应抗体的干扰。铜绿假单胞菌感染患者的抗体滴度较高，且根据感染阶段有所不同。这些初步结果表明，该测定法可能有助于评估囊性纤维化患者铜绿假单胞菌感染的状况。
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引用本文的文献
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Serum IgA antibodies against Pseudomonas aeruginosa in cystic fibrosis.囊性纤维化患者血清中针对铜绿假单胞菌的IgA抗体
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IgG antibodies in early Pseudomonas aeruginosa infection in cystic fibrosis.囊性纤维化患者早期铜绿假单胞菌感染中的IgG抗体
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Measurement of human antibody activity against Escherichia coli and Pseudomonas aeruginosa using formalin treated whole organisms in an ELISA technique.采用酶联免疫吸附测定（ELISA）技术，使用经福尔马林处理的全菌体来检测人针对大肠杆菌和铜绿假单胞菌的抗体活性。
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The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity.酶联免疫吸附测定（ELISA）：一种抗体浓度或亲和力的测量方法。
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