开发酶联免疫吸附测定法（ELISA）以检测囊性纤维化患者血清中针对铜绿假单胞菌细胞表面抗原的抗体。
Development of enzyme linked immunosorbent assay (ELISA) to detect antibodies to Pseudomonas aeruginosa cell surface antigens in sera of patients with cystic fibrosis.
作者信息
Brett M M, Ghoneim A T, Littlewood J M, Losowsky M S
出版信息
J Clin Pathol. 1986 Oct;39(10):1124-9. doi: 10.1136/jcp.39.10.1124.
Abstract
An enzyme linked immunosorbent assay (ELISA) to measure free serum IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis was developed. Seven strains of P aeruginosa cells, treated with glutaraldehyde and representing the most commonly isolated serotypes in our cystic fibrosis unit, were used. The specificity of the test was confirmed by the absence of cross reacting antibodies to other Gram negative bacteria. The results showed differences in the titres of antibodies at different stages of P aeruginosa infection. Because of its reproducibility, specificity, and sensitivity these preliminary results suggest that this test may be of value in monitoring the progress of P aeruginosa infection in patients with cystic fibrosis.
摘要
开发了一种酶联免疫吸附测定法（ELISA），用于测量囊性纤维化患者血清中抗铜绿假单胞菌的游离IgG抗体。使用了七株经戊二醛处理的铜绿假单胞菌细胞，它们代表了我们囊性纤维化病房中最常分离出的血清型。该测试的特异性通过不存在与其他革兰氏阴性菌的交叉反应抗体得到证实。结果显示，在铜绿假单胞菌感染的不同阶段，抗体滴度存在差异。由于其可重复性、特异性和敏感性，这些初步结果表明该测试可能对监测囊性纤维化患者铜绿假单胞菌感染的进展有价值。
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