Siddiqui Nazish, Rauf Abdur, Latif Abdul, Mahmood Zeenat
Department of Ilmul Advia (Unani Pharmacological and Pharmaceutical Sciences), Faculty of Unani Medicine, Aligarh Muslim University, Aligarh, India.
J Taibah Univ Med Sci. 2017 Jan 13;12(4):360-363. doi: 10.1016/j.jtumed.2016.11.006. eCollection 2017 Aug.
This study quantitatively determined the total phenolic contents in ethanolic and aqueous extracts of Gul-e-Zoofa (flowers of Benth) using a spectrophotometric method. We also performed a spectral study (UV and IR) of the ethanolic extract and a fluorescence study of the powdered drug and successive extracts to identify and characterize the genuine herbal drug, which has not been previously performed.
The total phenolic content was determined quantitatively using the Folin Ciocalteu reagent, with Gallic acid as the standard. The fluorescence characteristics of the powdered drug and successive extracts with and without chemical treatment during the day and under a UV light were recorded. The UV and IR spectra of the alcoholic extract of Gul-e-Zoofa were recorded using a spectrometer.
The total phenolic contents of the alcoholic and aqueous extracts were found to be 326.28 and 319.14 mg/g of the Gallic acid equivalent (GAE), respectively. The wavelength of the maximum absorption in the UV spectrum was 320 nm, and the characteristic frequencies in the IR spectrum were 3465.31, 3220.07, 2927.3, 2856.1, 1709.07, 1610.19, 1404.5, 1250.2, 1056.42, 823.04, 775.58, 577.81, and 463.10 cm. The fluorescence characteristics of the powdered drug were also observed.
This spectral and fluorescence study of the drug will be helpful for confirming the identity and purity of the genuine drug. The total phenolic content will be helpful for developing new drugs and standardizing the drug. The presence of a high total phenolic content shows that the flowers of Benth may possess antioxidant properties, which could lead to a new field of research in the future.
本研究采用分光光度法对古尔-佐法（本氏马先蒿花）乙醇提取物和水提取物中的总酚含量进行了定量测定。我们还对乙醇提取物进行了光谱研究（紫外和红外），并对该草药粉末及连续提取物进行了荧光研究，以鉴定和表征这种尚未进行过此类研究的正宗草药。
以没食子酸为标准品，采用福林-酚试剂定量测定总酚含量。记录该草药粉末及连续提取物在白天和紫外灯下经化学处理和未经化学处理的荧光特性。使用光谱仪记录古尔-佐法乙醇提取物的紫外和红外光谱。
乙醇提取物和水提取物的总酚含量分别为326.28和319.14毫克/克没食子酸当量（GAE）。紫外光谱中的最大吸收波长为320纳米，红外光谱中的特征频率为3465.31、3220.07、2927.3、2856.1、1709.07、1610.19、1404.5、1250.2、1056.42、823.04、775.58、577.81和463.10厘米。还观察到了该草药粉末的荧光特性。
该草药的光谱和荧光研究将有助于确认正宗药物的身份和纯度。总酚含量将有助于开发新药和规范该药物。高总酚含量的存在表明本氏马先蒿花可能具有抗氧化特性，这可能会在未来开辟一个新的研究领域。
