Mohammad Hosseini Seyed, Hossein Moshrefi Amir, Amani Reza, Vahid Razavimehr Seyed, Hasan Aghajanikhah Mohammad, Sokouti Zahra, Babaei Holari Behnam
Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran.
Young Researchers and Elite Club, Babol Branch, Islamic Azad University, Babol, Iran.
Int J Reprod Biomed. 2019 Mar 19;17(2):107-18. doi: 10.18502/ijrm.v17i2.3988. eCollection 2019 Feb.
Zinc performs many biochemical and physiological functions; however, toxicological studies demonstrate that Nano-zinc oxide has harmful effects on human health and environmental species in high concentrations.
The aim of this study was to investigate the toxicity of zinc oxide nanoparticles on reproductive tissues of female rat.
Eighty female Wistar adult rats weighing 180-200 gr, divided into eight groups (n= 10 in each group) including control, sham (treated with saline), and six groups injected with different doses of zinc oxide nanoparticle with 10-30 nanometer size (4, 8, 25, 50, 100, and 200 mg/kg) twice a week for four weeks. At the end of the study, the rats were bled and slaughtered; the Ovary and Uterus were taken for histopathology studies and blood samples were transferred to the laboratory for biochemical analysis.
Microscopic diagnoses in ovary tissue were included; increase in the corpus luteum, follicular cysts, inflammatory cells infiltration and fibrosis. Histopathological changes in ovary in a dose-dependent manner. In uterus tissue the lesions consisted; epithelial destruction, hyperplasia of endometrial glands. The Estrogen and Progesterone level in the serum of rats increased in low doses and reduced in a dose-dependent manner at high doses.
The results of the current study proved the toxicity of zinc oxide nanoparticles on the ovary and uterus organs at high concentrations, so further investigation is needed to reduce these effects.
锌具有多种生化和生理功能；然而，毒理学研究表明，高浓度的纳米氧化锌对人类健康和环境物种具有有害影响。
本研究旨在探讨氧化锌纳米颗粒对雌性大鼠生殖组织的毒性。
80只体重180 - 200克的成年雌性Wistar大鼠，分为八组（每组n = 10），包括对照组、假手术组（用生理盐水处理），以及六组每周两次注射不同剂量（4、8、25、50、100和200毫克/千克）粒径为10 - 30纳米的氧化锌纳米颗粒，共四周。研究结束时，对大鼠进行采血并处死；取出卵巢和子宫进行组织病理学研究，血液样本送至实验室进行生化分析。
卵巢组织的显微镜诊断结果包括；黄体增加、卵泡囊肿、炎性细胞浸润和纤维化。卵巢的组织病理学变化呈剂量依赖性。子宫组织的病变包括；上皮破坏、子宫内膜腺体增生。大鼠血清中的雌激素和孕酮水平在低剂量时升高，在高剂量时呈剂量依赖性降低。
本研究结果证明高浓度的氧化锌纳米颗粒对卵巢和子宫器官具有毒性，因此需要进一步研究以减轻这些影响。
