Ribeiro Ana Raquel Carneiro, Cordeiro Maria Lúcia da Silva, Silva Larissa Marina Pereira, Cadavid Cesar Orlando Munoz, Caland Ricardo Basílio de Oliveira, Fernandes-Negreiros Marília Medeiros, Queiroz Moacir Fernandes, Barbosa Jefferson da Silva, Aragão Cicero Flavio Soares, Zucolotto Silvana Maria, Oliveira Riva de Paula, Rocha Hugo Alexandre Oliveira, Scortecci Kátia Castanho
Programa de Pós-Graduação em Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte-UFRN, Centro de Biociências, Natal, Rio Grande do Norte (RN) 59.072-970, Brazil.
Laboratório de Transformação de Plantas e Análise de Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Centro de Biociências, UFRN, Natal-RN 59.072-970, Brazil.
Antioxidants (Basel). 2019 Aug 15;8(8):310. doi: 10.3390/antiox8080310.
species are widely studied to identify their chemical composition and evaluate their biological activity. Since evidence supporting the potential antioxidant and antiproliferative activity of is lacking, the aim of this work was to evaluate these activities in six different leaf extracts: hexane (CHE), chloroform (CCE), ethanolic (CEE), methanolic (CME), aqueous final (CFAE), and only aqueous (CAE). The presence of phenolic compounds, tannin, saponin, and ursolic acid was determined by thin layer chromatography (TLC). CEE, CME, and CFAE showed in vitro antioxidant activity at the initiation, propagation, and termination stages of oxidative damage. Moreover, no toxicity was observed in the 3T3 non-cancerous cell line. On the other hand, all extracts promoted cell death in the tumor cell lines human cervical adenocarcinoma cell line (HeLa) and human stomach gastric adenocarcinoma cell line (AGS). Based on these results, the effect of CEE on the AGS cell line was analyzed using flow cytometry, and necrosis and late apoptosis were observed. Finally, the model showed that CEE was able to reduce the basal reactive oxygen species (ROS) level. Ultra-performance liquid chromatography (UPLC) analysis showed rutin as the major compound in CEE. Therefore, fresh leaves may be potential sources of molecules possessing antioxidant and antiproliferative activities.
人们对多种物种进行了广泛研究，以确定其化学成分并评估其生物活性。由于缺乏支持[具体物种]潜在抗氧化和抗增殖活性的证据，本研究的目的是评估六种不同叶提取物的这些活性：己烷提取物（CHE）、氯仿提取物（CCE）、乙醇提取物（CEE）、甲醇提取物（CME）、最终水提取物（CFAE）和仅水提取物（CAE）。通过薄层色谱法（TLC）测定酚类化合物、单宁、皂苷和熊果酸的存在情况。CEE、CME和CFAE在氧化损伤的起始、传播和终止阶段均表现出体外抗氧化活性。此外，在3T3非癌细胞系中未观察到毒性。另一方面，所有提取物均能促进肿瘤细胞系人宫颈腺癌细胞系（HeLa）和人胃腺癌细胞系（AGS）的细胞死亡。基于这些结果，使用流式细胞术分析了CEE对AGS细胞系的影响，观察到坏死和晚期凋亡。最后，[具体模型]表明CEE能够降低基础活性氧（ROS）水平。超高效液相色谱（UPLC）分析表明芦丁是CEE中的主要化合物。因此，[具体物种]鲜叶可能是具有抗氧化和抗增殖活性分子的潜在来源。
