Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Talanta. 2019 Dec 1;205:120140. doi: 10.1016/j.talanta.2019.120140. Epub 2019 Jul 9.
Herein, we synthesized a kind of conductive supramolecular polymer hydrogel (CSPH) based on polyaniline (PANI) which can not only improve the conductivity but also promote antifouling performance of the aptasensor for the specific recruitment of thrombin (TB) from complex samples. With the electrochemical copolymerization of aniline (AN) and 3-aminophenylboronic acid (ABA) on glassy carbon electrode (GCE), the electrode was then inserted into the polyvinyl alcohol (PVA) solution to obtain robust CSPH through boric acid groups incorporated onto PANI to cause gelation of PVA solution, owing to the hydrophilicity of CSPH and nearly electrical neutrality, the modified electrode is antifouling without integration of other antifouling materials. A sandwich-type electrochemical aptasensor was constructed on the CSPH based electrode interfaces. Thrombin aptamer 1 (TBA1) were modified on the CSPH through amide bond, and thrombin aptamer 2 modified magnetic nanoparticles (MNP-TBA2) are used as signal amplification probes, the aptasensor has good sensitivity with a linear range from 1 pmol/L to 10 nmol/L and has a detection limit down to 0.64 pmol/L. The strategy of utilizing eletropolymerization of CSPH films to undergo highly selective thrombin recognition is, of course, readily extended to a broad range of targets in the real samples, and the recovery was ranging from 95.2% to 106.3% and RSDs varying from 2.3% to 4.5%.
在此，我们合成了一种基于聚苯胺 (PANI) 的导电超分子聚合物水凝胶 (CSPH)，它不仅可以提高导电性，还可以促进适体传感器的抗污染性能，从而从复杂样品中特异性募集凝血酶 (TB)。通过在玻碳电极 (GCE) 上电化学共聚苯胺 (AN) 和 3-氨基苯硼酸 (ABA)，然后将电极插入聚乙烯醇 (PVA) 溶液中，通过掺入 PANI 上的硼酸基团使 PVA 溶液凝胶化，从而获得坚固的 CSPH，由于 CSPH 的亲水性和几乎电中性，修饰后的电极无需集成其他抗污染材料即可抗污染。在基于 CSPH 的电极界面上构建了三明治型电化学适体传感器。通过酰胺键将凝血酶适体 1 (TBA1) 修饰在 CSPH 上，并且将修饰有凝血酶适体 2 的磁性纳米粒子 (MNP-TBA2) 用作信号放大探针，该适体传感器具有良好的灵敏度，线性范围从 1 pmol/L 到 10 nmol/L，检测限低至 0.64 pmol/L。利用 CSPH 薄膜的电聚合进行高选择性凝血酶识别的策略当然可以很容易地扩展到实际样品中的广泛目标，回收率范围为 95.2%至 106.3%，RSD 从 2.3%到 4.5%不等。
