小鼠脑内β-淀粉样蛋白淋巴清除的经颅光生物调节的初步研究：阿尔茨海默病非药物治疗的突破性策略
Pilot study of transcranial photobiomodulation of lymphatic clearance of beta-amyloid from the mouse brain: breakthrough strategies for non-pharmacologic therapy of Alzheimer's disease.
作者信息
Zinchenko Ekaterina, Navolokin Nikita, Shirokov Alexander, Khlebtsov Boris, Dubrovsky Alexander, Saranceva Elena, Abdurashitov Arkady, Khorovodov Alexander, Terskov Andrey, Mamedova Aysel, Klimova Maria, Agranovich Ilana, Martinov Dmitry, Tuchin Valery, Semyachkina-Glushkovskaya Oxana, Kurts Jurgen
机构信息
Saratov State University, Astrakhanskaya Str. 83, Saratov 410012, Russia.
Saratov State Medical University, Saratov 410012, Russia.
出版信息
Biomed Opt Express. 2019 Jul 15;10(8):4003-4017. doi: 10.1364/BOE.10.004003. eCollection 2019 Aug 1.
Abstract
In this pilot study, we analyzed effects of transcranial photobiomodulation (tPBM, 1267 nm, 32 J/cm) on clearance of beta-amyloid (Aβ) from the mouse brain. The immunohistochemical and confocal data clearly demonstrate the significant reduction of deposition of Aβ plaques in mice after tPBM vs. untreated animals. The behavior tests showed that tPBM improved the cognitive, memory and neurological status of mice with Alzheimer's disease (AD). Using of our original method based on optical coherence tomography (OCT) analysis of clearance of gold nanorods (GNRs) from the brain, we proposed possible mechanism underlying tPBM-stimulating effects on clearance of Aβ via the lymphatic system of the brain and the neck. These results open breakthrough strategies for a non-pharmacological therapy of Alzheimer's disease and clearly demonstrate that tPBM might be a promising therapeutic target for preventing or delaying Alzheimer's disease.
摘要
在这项初步研究中，我们分析了经颅光生物调节（tPBM，1267纳米，32焦耳/平方厘米）对小鼠脑内β-淀粉样蛋白（Aβ）清除的影响。免疫组织化学和共聚焦数据清楚地表明，与未治疗的动物相比，tPBM处理后的小鼠脑内Aβ斑块沉积显著减少。行为测试表明，tPBM改善了阿尔茨海默病（AD）小鼠的认知、记忆和神经状态。通过我们基于光学相干断层扫描（OCT）分析脑内金纳米棒（GNRs）清除情况的原始方法，我们提出了tPBM通过脑和颈部淋巴系统刺激Aβ清除的潜在机制。这些结果为阿尔茨海默病的非药物治疗开辟了突破性策略，并清楚地表明tPBM可能是预防或延缓阿尔茨海默病的一个有前景的治疗靶点。
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