Altimira Fabiola, De La Barra Nathalia, Rebufel Patricia, Soto Sylvana, Soto Rodrigo, Estay Patricia, Vitta Nancy, Tapia Eduardo
Laboratory of Entomology, Instituto de Investigaciones Agropecuarias, INIA La Platina, Santa Rosa 11610, La Pintana, Santiago, Chile.
Laboratory of Phytopathology, Instituto de Investigaciones Agropecuarias, INIA La Platina, Santa Rosa 11610, La Pintana, Santiago, Chile.
BMC Res Notes. 2019 Aug 28;12(1):548. doi: 10.1186/s13104-019-4584-6.
Lobesia botrana, the European grapevine moth, affects Vitis vinifera L. and other species of economic importance in a number of countries through damage caused by its larvae in berries and associated secondary diseases such as Botrytis cinerea. Control of the moth in urban areas is difficult due to poor chemical management of infested plants in houses. Additionally, in winter, L. botrana is in its pupal stage covered with a cocoon that prevents the penetration of chemical pesticides. For this reason, the objective of this work was to control the pupal stage with a formulation based on the entomopathogenic fungus Beauveria pseudobassiana in urban areas.
The strain RGM 1747 was identified as B. pseudobassiana by multilocus sequence analysis. The biocontrol activity of this formulated fungus against the infestation of vines with breeding pupae without cocoons showed 100% infection 21 days after inoculation under winter conditions. Finally, the biocontrol activity of the formulated fungus against natural infestations of L. botrana in winter in urban areas reached an efficacy of 51%. This result suggests that the B. pseudobassiana formulation is able to penetrate the cocoon and contributes to the integrated pest management of L. botrana.
欧洲葡萄蛾葡萄浆果小卷蛾会对酿酒葡萄及其他一些具有经济重要性的物种造成影响，其幼虫会损害浆果，并引发诸如灰霉病等相关次生病害，在许多国家均有发生。由于家庭中受侵染植物的化学防治效果不佳，城市地区对这种蛾类的防治较为困难。此外，在冬季，葡萄浆果小卷蛾处于蛹期，被茧包裹，这会阻止化学农药的渗透。因此，本研究的目的是在城市地区使用基于昆虫病原真菌拟白僵菌的制剂来防治蛹期害虫。
通过多位点序列分析，菌株RGM 1747被鉴定为拟白僵菌。在冬季条件下，这种制剂真菌对无茧繁殖蛹侵染葡萄藤的生物防治活性在接种后21天显示出100%的感染率。最后，该制剂真菌在城市地区冬季对葡萄浆果小卷蛾自然侵染的生物防治活性达到了51%的防治效果。这一结果表明，拟白僵菌制剂能够穿透茧，并有助于葡萄浆果小卷蛾的综合虫害管理。
