Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), National Jiangsu Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing, 210023, China.
Weed Research Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
Biotechnol J. 2020 Jan;15(1):e1800741. doi: 10.1002/biot.201800741. Epub 2019 Sep 25.
Plasmonic nanomaterials, especially Au and Ag nanomaterials, have shown attractive physicochemical properties, such as easy functionalization and tunable optical bands. The development of this active subfield paves the way to the fascinating biosensing platforms. In recent years, plasmonic nanomaterials-based sensors have been extensively investigated because they are useful for genetic diseases, biological processes, devices, and cell imaging. In this account, a brief introduction of the development of optical biosensors based on DNA-functionalized plasmonic nanomaterials is presented. Then the common strategies for the application of the optical sensors are summarized, including colorimetry, fluorescence, localized surface plasmon resonance, and surface-enhanced resonance scattering detection. The focus is on the fundamental aspect of detection methods, and then a few examples of each method are highlighted. Finally, the opportunities and challenges for the plasmonic nanomaterials-based biosensing are discussed with the development of modern technologies.
等离子体纳米材料，尤其是金和银纳米材料，表现出了有吸引力的物理化学性质，例如易于功能化和可调光学波段。这一活跃的子领域的发展为迷人的生物传感平台铺平了道路。近年来，基于等离子体纳米材料的传感器得到了广泛的研究，因为它们对遗传疾病、生物过程、器件和细胞成像很有用。在本报告中，简要介绍了基于 DNA 功能化等离子体纳米材料的光学生物传感器的发展。然后总结了光学传感器应用的常见策略，包括比色法、荧光法、局域表面等离子体共振和表面增强共振散射检测。重点是检测方法的基础方面，然后突出每种方法的几个例子。最后，随着现代技术的发展，讨论了基于等离子体纳米材料的生物传感的机遇和挑战。
