Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Experimental Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
Adv Exp Med Biol. 2019;1155:507-520. doi: 10.1007/978-981-13-8023-5_47.
Asthenospermia has been considered as one of the crucial causes of male infertility, which was closely related to epididymal dysfunction. Lots of documents have revealed that taurine palys an important role in male reproduction, including antioxidation, membrane stabilization, stimulation of sexual hormone secretion and elevation of sperm quality. The objective of this study was to expose the effect of taurine on spermatozoa quality and function in ornidazole-induced asthenospermia rats. We found that taurine treatment could obviously recover the decline of cauda epididymal sperm count, viability and motility, and the elevation of sperm abnormality in asthenospermia animals. Spermatozoa acrosin, LDH-X, SDH and CCO activities of model rats also were notably increased by taurine administration. The present data indicated that taurine could raise spermatozoa quality and function by elevating mitochondrial energy metabolism. Notably, taurine supplementation markedly raised serum GnRH, LH and T levels in asthenospermia rays, suggesting taurine rescued asthenosperm by means of stimulating hypothalamic-pituitary-testicular axis secretion. We also found that concentrations of asthenospermia epididymal carnitine, SA, α-Glu and ACP, and mRNA expression levels of MMP7 and IDO2 were significantly rised by taurine administration, indicating taurine may protect epididymal epithelium structure, improve secretion activity, and maintain intraluminal microenvironment homeostasis. Finally, the present results showed taurine effectively increased cauda epididymal SOD, GSH and γ-GT levels in model rats, reduced ROS and MDA production, suggesting epididymal antioxidant ability of asthenospermia rats could be elevated by taurine treatment. To sum up, our results indicated that taurine can promote spermatozoa quality and function in ornidazole-induced asthenospermia rats by facilitating epididymal epithelium secretion and luminal microenvironment homeostasis.
弱精子症被认为是男性不育的关键原因之一，其与附睾功能障碍密切相关。大量文献表明，牛磺酸在男性生殖中发挥着重要作用，包括抗氧化、膜稳定、刺激性激素分泌和提高精子质量。本研究旨在探讨牛磺酸对奥硝唑诱导的弱精子症大鼠精子质量和功能的影响。结果发现，牛磺酸治疗可明显恢复弱精子症动物附睾尾部精子计数、活力和运动能力的下降以及精子畸形率的升高。牛磺酸给药还显著增加了模型大鼠精子顶体酶、LDH-X、SDH 和 CCO 活性。这些数据表明，牛磺酸通过提高线粒体能量代谢来提高精子质量和功能。值得注意的是，牛磺酸补充剂可显著提高弱精子症射线的血清 GnRH、LH 和 T 水平，表明牛磺酸通过刺激下丘脑-垂体-睾丸轴分泌来挽救弱精子症。我们还发现，弱精子症附睾肉碱、SA、α-Glu 和 ACP 的浓度以及 MMP7 和 IDO2 的 mRNA 表达水平均显著升高，表明牛磺酸可能保护附睾上皮结构，提高分泌活性，维持管腔内微环境稳态。最后，本研究结果表明，牛磺酸可有效增加模型大鼠附睾尾部 SOD、GSH 和 γ-GT 水平，降低 ROS 和 MDA 的产生，表明牛磺酸治疗可提高弱精子症大鼠附睾的抗氧化能力。总之，本研究结果表明，牛磺酸通过促进附睾上皮细胞分泌和管腔内微环境稳态，可提高奥硝唑诱导的弱精子症大鼠的精子质量和功能。
