Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China.
Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China.
Mar Drugs. 2019 Aug 29;17(9):509. doi: 10.3390/md17090509.
XT20-134 (MCCC 3A00956) is a fungus with cytotoxic activity, derived from deep-sea sediment. Five new compounds, adeninylpyrenocine (), 2-hydroxyl-3-pyrenocine-thio propanoic acid (), ozazino--(2,3-dihydroxyl-trp-tyr) (), 5,5-dichloro-1-(3,5-dimethoxyphenyl)-1,4-dihydroxypentan-2-one (), and 2,3,4-trihydroxybutyl cinnamate (), together with 19 known compounds (-), were isolated from an ethyl acetate (EtOAc) extract of its fermentation. The structures of the new compounds were comprehensively characterized by high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS), 1D and 2D nuclear magnetic resonance (NMR). All isolates were evaluated for their cytotoxic activities. The heteroatom-containing new compounds and showed potent cytotoxicity to the human hepatoma tumor cell Bel7402 with IC values of 7.63 ± 1.46, 13.14 ± 1.41 μM and the human fibrosarcoma tumor cell HT1080 with IC values of 10.22 ± 1.32, 16.53 ± 1.67 μM, respectively.
XT20-134（MCCC 3A00956）是一种具有细胞毒性的真菌，来源于深海沉积物。从其发酵的乙酸乙酯提取物中分离得到了五个新化合物：腺嘌呤基吡咯并[2,3-f]喹嗪（）、2-羟基-3-吡咯并喹啉硫代丙酸（）、ozazino-[2,3-二羟基-Trp-Tyr]（）、5,5-二氯-1-(3,5-二甲氧基苯基)-1,4-二羟基戊-2-酮（）和 2,3,4-三羟基丁基肉桂酸酯（），以及 19 个已知化合物（-）。新化合物的结构通过高分辨率电喷雾电离质谱（HR-ESI-MS）、1D 和 2D 核磁共振（NMR）进行了全面表征。所有分离物均进行了细胞毒性活性评估。含杂原子的新化合物和表现出对人肝癌肿瘤细胞 Bel7402 的强烈细胞毒性，IC 值分别为 7.63±1.46 μM 和 13.14±1.41 μM，对人纤维肉瘤肿瘤细胞 HT1080 的 IC 值分别为 10.22±1.32 μM 和 16.53±1.67 μM。
