Dental School, Colegio Máximo, Research Institute IBS, University of Granada, Campus de Cartuja s/n, 18017, Granada, Spain.
Faculty of Dentistry, Oral Surgery Section, University of Sevilla, Avicena s/n, 41009, Sevilla, Spain.
Clin Oral Investig. 2020 Jun;24(6):2037-2049. doi: 10.1007/s00784-019-03068-8. Epub 2019 Sep 6.
The aim of this study was to evaluate the bone-regeneration efficiency of novel polymeric nanostructured membranes and the effect of zinc, calcium, titanium, and bone morpho-protein loading on membranes, through an in vivo rabbit model.
Nanostructured membranes of methylmethacrylate were loaded with zinc, calcium, TiO nanoparticles, and bone-morphogenetic protein (BMP). These membranes covered the bone defects prepared on the skulls of six rabbits. Animals were sacrificed 6 weeks after surgery. Micro computed tomography was used to evaluate bone architecture through BoneJ pluging and ImageJ script. Three histological processing of samples, including von Kossa silver nitrate, toluidine blue, and fluorescence by the deposition of calcein were utilized.
Zn-membranes (Zn-Ms) promoted the highest amount of new bone and higher bone perimeter than both unloaded and Ti-membranes (Ti-Ms). Ca-membranes (Ca-Ms) attained higher osteoid perimeter and bone perimeter than Zn-Ms. The skeleton analysis showed that Zn-Ms produced more branches and junctions at the trabecular bone than BMP-loaded membranes (BMP-Ms). Samples treated with Ti-Ms showed less bone formation and bony bridging processes. Both Zn-Ms and Ca-Ms achieved higher number of osteoblasts than the control group. BMP-Ms and Ca-Ms originated higher number of blood vessels than Ti-Ms and control group.
Zn incorporation in novel nanostructured membranes provided the highest regenerative efficiency for bone healing at the rabbit calvarial defects.
Zn-Ms promoted osteogenesis and enhanced biological activity, as mineralized and osteoid new bone with multiple interconnected ossified trabeculae appeared in close contact with the membrane.
本研究旨在通过兔颅骨骨缺损模型，评估新型聚合纳米结构膜的骨再生效率，以及锌、钙、钛和骨形态蛋白负载对膜的影响。
甲基丙烯酸甲酯纳米结构膜负载锌、钙、TiO 纳米粒子和骨形态发生蛋白（BMP）。这些膜覆盖在 6 只兔子颅骨上制备的骨缺损处。术后 6 周处死动物。采用微计算机断层扫描技术，通过 BoneJ 插件和 ImageJ 脚本评估骨结构。对样本进行了 3 种组织学处理，包括硝酸银 von Kossa、甲苯胺蓝和钙沉积的荧光。
锌膜（Zn-Ms）比未负载膜和钛膜（Ti-Ms）促进了更多的新骨形成和更高的骨周径。钙膜（Ca-Ms）的类骨质周径和骨周径高于 Zn-Ms。骨骼分析表明，Zn-Ms 在小梁骨上产生的分支和连接比负载 BMP 的膜（BMP-Ms）更多。用 Ti-Ms 处理的样本显示出较少的骨形成和骨桥接过程。Zn-Ms 和 Ca-Ms 产生的成骨细胞数量均高于对照组。BMP-Ms 和 Ca-Ms 产生的血管数量多于 Ti-Ms 和对照组。
新型纳米结构膜中锌的掺入为兔颅骨缺损的骨愈合提供了最高的再生效率。
Zn-Ms 促进了成骨作用并增强了生物活性，因为矿化和类骨质新骨与膜紧密接触，出现了多个相互连接的骨化小梁。
