Abdullah Nurul Aliah, Ja'afar Fairuzeta, Yasin Hartini M, Taha Hussein, Petalcorin Mark I R, Mamit Mitasby H, Kusrini Eny, Usman Anwar
Department of Chemistry, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam.
Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam.
Heliyon. 2019 Sep 14;5(9):e02476. doi: 10.1016/j.heliyon.2019.e02476. eCollection 2019 Sep.
In this study, the physicochemical, antioxidant, antibacterial properties, and the toxicity of propolis particles produced by stingless bee found in Brunei Darussalam were investigated. Propolis particles of different sizes were extracted from raw propolis using various volume fractions of ethanol in water. Spectroscopic analyses were utilized to characterize the chemical structures, functional groups, as well as absorbance and fluorescence properties. The total antioxidant capacity of propolis particles, which was assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, was found to increase with volume fraction of ethanol. The maximum antioxidant capacity was as high as 317.65 mg ascorbic acid equivalent per gram of propolis particles. All of the propolis particles showed antibacterial activity against Gram-positive ( and ) and Gram-negative bacteria ( and ). The diameters of the inhibition zone were either significantly higher or equivalent to those of two standard antibiotics (rifampicin and streptomycin), suggesting strong antibacterial activity. The toxicity studies of propolis particles against revealed that they are non-toxic after 24 h exposure. Overall findings suggest that propolis particles are not only an important source of natural antioxidants that could be beneficial for human health, but they have potentials as antimicrobial against bacteria.
在本研究中，对文莱达鲁萨兰国发现的无刺蜂所产蜂胶颗粒的物理化学性质、抗氧化性能、抗菌性能及毒性进行了研究。使用不同体积分数的乙醇水溶液从生蜂胶中提取出不同尺寸的蜂胶颗粒。利用光谱分析来表征化学结构、官能团以及吸光度和荧光特性。通过DPPH（2,2-二苯基-1-苦基肼）法评估的蜂胶颗粒总抗氧化能力，发现其随乙醇体积分数的增加而提高。最大抗氧化能力高达每克蜂胶颗粒317.65毫克抗坏血酸当量。所有蜂胶颗粒对革兰氏阳性菌（ 和 ）和革兰氏阴性菌（ 和 ）均表现出抗菌活性。抑菌圈直径显著高于或等同于两种标准抗生素（利福平和链霉素）的抑菌圈直径，表明其具有较强的抗菌活性。蜂胶颗粒对 的毒性研究表明，暴露24小时后它们无毒。总体研究结果表明，蜂胶颗粒不仅是对人体健康有益的天然抗氧化剂的重要来源，而且具有作为抗菌剂对抗细菌的潜力。
