Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06, Malmö, Sweden.
Biofilms - Research Center for Biointerfaces, Malmö University, SE-205 06, Malmö, Sweden.
Sci Rep. 2019 Sep 10;9(1):12948. doi: 10.1038/s41598-019-49245-3.
Massive integration of biosensors into design of Internet-of-Things (IoT) is vital for progress of healthcare. However, the integration of biosensors is challenging due to limited availability of battery-less biosensor designs. In this work, a combination of nanomaterials for wireless sensing of biological redox reactions is described. The design exploits silver nanoparticles (AgNPs) as part of the RFID tag antenna. We demonstrate that a redox enzyme, particularly, horseradish peroxidase (HRP), can convert AgNPs into AgCl in the presence of its substrate, hydrogen peroxide. This strongly changes the impedance of the tag. The presented example exploits gold nanoparticle (AuNP)-assisted electron transfer (ET) between AgNPs and HRP. We show that AuNP is a vital intermediate for establishing rapid ET between the enzyme and AgNPs. As an example, battery-less biosensor-RFID tag designs for HO and glucose are demonstrated. Similar battery-less sensors can be constructed to sense redox reactions catalysed by other oxidoreductase enzymes, their combinations, bacteria or other biological and even non-biological catalysts. In this work, a fast and general route for converting a high number of redox reaction based sensors into battery-less sensor-RFID tags is described.
大规模地将生物传感器集成到物联网 (IoT) 的设计中对于医疗保健的发展至关重要。然而，由于缺乏无电池生物传感器设计，因此集成生物传感器具有挑战性。在这项工作中，描述了用于生物氧化还原反应无线感应的纳米材料的组合。该设计利用银纳米颗粒 (AgNP) 作为 RFID 标签天线的一部分。我们证明，在其底物过氧化氢存在的情况下，氧化还原酶，特别是辣根过氧化物酶 (HRP)，可以将 AgNP 转化为 AgCl。这强烈改变了标签的阻抗。所提出的示例利用金纳米颗粒 (AuNP) 辅助 AgNP 和 HRP 之间的电子转移 (ET)。我们表明，AuNP 是在酶和 AgNP 之间建立快速 ET 的重要中间物。例如，展示了用于 HO 和葡萄糖的无电池生物传感器-RFID 标签设计。可以构建类似的无电池传感器来感测其他氧化还原酶、它们的组合、细菌或其他生物甚至非生物催化剂催化的氧化还原反应。在这项工作中，描述了将大量基于氧化还原反应的传感器快速且普遍地转换为无电池传感器-RFID 标签的方法。
