Moubayed Nadine M S, Al Houri Hadeel Jawad, Al Khulaifi Manal M, Al Farraj Dunia A
King Saud University, Sciences College, Microbiology Department, Girls Campus, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Saudi J Biol Sci. 2017 Jan;24(1):162-169. doi: 10.1016/j.sjbs.2016.05.018. Epub 2016 Jun 16.
The present study demonstrates the antibacterial activity of selected brown and green marine algae collected from Saudi Arabia Red Sea and Arabian Gulf. The methanolic and acetone extracts were tested against gram positive, gram negative bacteria and in an attempt to be used as an alternative to commonly used antibiotics. Both brown seaweed species and methanolic extracts were found to be active against gram positive than gram negative; however, acetone extract gave the highest inhibitory activity against sp. On the other hand, organic extract demonstrated higher antibacterial activity than the fresh extract but both extracts revealed decreased activity compared to extracts. methanolic extract showed an obvious effect on methicillin resistant (MRSA). The present work shows a comparable therapeutic potency of the tested seaweed members and extracts in treating human microbial pathogens to synthetic chemical antibiotics. A remarkable higher antioxidant DPPH free radical scavenging effect was recorded with sp. compared to sp. FTIR Infrared Spectrometer analysis together with the high performance liquid chromatography provided a detailed description of the possible functional constituents and the major chemical components present in marine macroalgae particularly in brown seaweeds to be mainly of phenolic nature to which the potent antimicrobial activity is being attributed.
本研究展示了从沙特阿拉伯红海和阿拉伯湾采集的特定褐藻和绿藻的抗菌活性。对甲醇提取物和丙酮提取物进行了针对革兰氏阳性菌、革兰氏阴性菌的测试，并试图将其用作常用抗生素的替代品。发现两种褐藻物种的甲醇提取物对革兰氏阳性菌的活性高于革兰氏阴性菌；然而，丙酮提取物对[具体菌种]的抑制活性最高。另一方面，有机提取物的抗菌活性高于新鲜提取物，但两种提取物与[其他提取物]相比活性均有所降低。[某种藻类]的甲醇提取物对耐甲氧西林金黄色葡萄球菌（MRSA）显示出明显效果。目前的研究表明，受试海藻及其提取物在治疗人类微生物病原体方面具有与合成化学抗生素相当的治疗效力。与[另一种藻类]相比，[某种藻类]的抗氧化二苯基苦味酰基自由基清除效果显著更高。傅里叶变换红外光谱仪分析以及高效液相色谱法详细描述了大型海藻尤其是褐藻中可能存在的功能成分和主要化学成分，其主要为酚类性质，强效抗菌活性归因于此。
