Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227 Cidade Universitária, São Paulo, 05508-000, SP, Brazil.
Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Freiburgstrasse 7 CH-3010, Bern, Switzerland.
Arch Oral Biol. 2020 Jan;109:104549. doi: 10.1016/j.archoralbio.2019.104549. Epub 2019 Sep 5.
This in vitro study aimed to evaluate different periods of exposure to clarified human saliva for the ability to protect enamel against erosive tooth wear.
For this purpose, sixty specimens (4 × 4 × 1.5 mm) were prepared from third human molars. For all groups, the period before abrasion was performed by remineralisation with human saliva (except in G1). The specimens were randomly divided into six groups (n = 10) according to the different remineralisation times of exposure to clarified human saliva: no exposure to saliva (G1) and 30 min (G2), 60 min (G3), 90 min (G4), 120 min (G5), and 240 min (G6) of exposure to human saliva. A 5-day cycling was performed with 5 min of erosion (1% citric acid; pH 2.3), 4x/day. After the first and last erosive episodes, the abrasion challenge was performed with slurry of fluoride toothpaste (1450 ppm F as sodium monofluorophosphate) plus human saliva (1:3), with an electric toothbrush (15 s, with a total of 120 s of slurry immersion). Surface loss (SL) was determined using an optical profilometer (n = 10) and for qualitative analysis, environmental scanning electron microscopy (ESEM) was performed (n = 3). The SL data were statistically analysed by one-way analysis of variance (α = 0.05).
No significant differences were detected among the groups for SL (p > 0.05), and ESEM showed similar aspects of eroded enamel.
The period of in vitro exposure to clarified human saliva was not able to protect against enamel erosion.
本体外研究旨在评估不同时期接触澄清人唾液对保护牙釉质免受侵蚀性牙齿磨损的能力。
为此，从第三磨牙中制备了六十个标本（4×4×1.5mm）。对于所有组，在进行磨损之前，通过人唾液（除 G1 组外）进行再矿化。根据暴露于澄清人唾液的不同再矿化时间，将标本随机分为六组（n=10）：无唾液暴露（G1）和 30 分钟（G2）、60 分钟（G3）、90 分钟（G4）、120 分钟（G5）和 240 分钟（G6）。进行为期 5 天的循环，每天 4 次，每次 5 分钟的侵蚀（1%柠檬酸；pH 值 2.3）。在第一次和最后一次侵蚀期之后，使用含氟牙膏（作为单氟磷酸钠的 1450ppm F）加人唾液（1：3）的浆料进行磨损挑战，使用电动牙刷（15s，总浸入浆料 120s）。使用光学轮廓仪（n=10）测定表面损失（SL），并进行定性分析，使用环境扫描电子显微镜（ESEM）（n=3）。通过单向方差分析（α=0.05）对 SL 数据进行统计学分析。
各组之间的 SL 无显著差异（p>0.05），ESEM 显示侵蚀牙釉质的相似方面。
体外暴露于澄清人唾液的时间不能防止牙釉质侵蚀。
