Adesina Tomilola, Nwinyi Obinna, De Nandita, Akinnola Olayemi, Omonigbehin Emmanuel
Department of Microbiology, College of Science and Technology, Covenant University, 112103 Ota, Nigeria.
Pathogens. 2019 Sep 25;8(4):164. doi: 10.3390/pathogens8040164.
Recently discovered extraintestinal obtained from non-clinical samples has exhibited the potential for acquiring multiple beta-lactamase genes, just like many extraintestinal strains. Albeit, they are often omitted or classified as . This study aimed to, therefore, identify carbapenem-resistant extended-spectrum beta-lactamase (ESBL) producing isolates from clinical samples, determine their evolutionary relatedness using 16S rRNA sequencing analysis and screen for beta-lactamase genes. A total of 135 septic wound samples were obtained from patients on referral at a General Hospital in Lagos, Nigeria. For the phenotypic identification of isolates from culture-positive samples, morphological, and physiological tests were carried out. Identities of the isolates harbouring beta-lactamase genes were assigned to their genus strains using the 16S rRNA sequencing. The Kirby Bauer disc diffusion technique and double-disc synergy test were used to screen isolates for multidrug resistance and ESBL production. Carbapenem-resistant ESBL producing isolates were screened for beta-lactamase genes in a polymerase chain reaction. Three isolates (CR11, CR35 and CR49) were obtained during this study. strains were motile, non-lactose and non-sorbitol fermenting but positive for cellobiose and adonitol fermentation. The I6S rRNA assigned the phenotypically identified isolates to species. All three isolates were multidrug-resistant, carbapenem-resistant and ESBL producers. Isolates CR11 and CR35 harboured cefotaximase (CTX-M) and temoniera (TEM) beta-lactamase genes while CR49 harboured sulfhydryl variable (SHV) beta-lactamase gene. We herein report the detection of multiple beta-lactamase genes in carbapenem-resistant ESBL producing from clinical samples.
最近从非临床样本中发现的肠外菌株已表现出获得多个β-内酰胺酶基因的潜力，就像许多肠外菌株一样。尽管如此，它们常常被忽略或归类为……。因此，本研究旨在从临床样本中鉴定产碳青霉烯耐药超广谱β-内酰胺酶（ESBL）的肠杆菌科分离株，使用16S rRNA测序分析确定它们的进化相关性，并筛选β-内酰胺酶基因。从尼日利亚拉各斯一家综合医院转诊的患者中总共获取了135份脓毒性伤口样本。对于培养阳性样本中分离株的表型鉴定，进行了形态学和生理学测试。使用16S rRNA测序将携带β-内酰胺酶基因的分离株的身份确定到其属菌株。采用 Kirby Bauer 纸片扩散技术和双纸片协同试验筛选分离株的多重耐药性和ESBL产生情况。在聚合酶链反应中筛选产碳青霉烯耐药ESBL的分离株中的β-内酰胺酶基因。在本研究中获得了三株肠杆菌科分离株（CR11、CR35和CR49）。肠杆菌科菌株具有运动性，不发酵乳糖和山梨醇，但纤维二糖和阿东糖醇发酵呈阳性。16S rRNA将表型鉴定的分离株鉴定为肠杆菌属物种。所有三株分离株均具有多重耐药性、碳青霉烯耐药性且产ESBL。分离株CR11和CR35携带头孢噻肟酶（CTX-M）和替莫西林（TEM）β-内酰胺酶基因，而CR49携带巯基可变（SHV）β-内酰胺酶基因。我们在此报告从临床样本中检测到产碳青霉烯耐药ESBL的肠杆菌科菌株中存在多个β-内酰胺酶基因。
