Tanay A, Tartakowsky B, Segal S, Yust I, Greif J, Topilsky M
Isr J Med Sci. 1985 Jan;21(1):6-11.
Using a method based on the differential affinity of mononuclear cells to insolubilized histamine, we were able to distinguish several differences in the composition of T cell subpopulations between healthy control subjects and sarcoidosis patients. In 5 of 10 patients with sarcoidosis, the ability of T cells to respond to three mitogens was severely depressed, while in the remaining 5 it was similar to the response of normal subjects. After the elimination of a subpopulation of peripheral blood mononuclear cells (PBMC) that adhered to the histamine column, the response to mitogens of the unbound cells of normal control subjects was significantly lower than values obtained before separation. The histamine-unbound lymphocytes of the five patients with active sarcoidosis, whose unseparated PBMC responded poorly to mitogens, reacted significantly better to the mitogens than before the separation. This pattern of responsiveness has been previously only in PBMC of patients with autoimmune diseases. It seems that some patients with sarcoidosis possess a subpopulation of T-lymphocytes with suppressor activity and a specific affinity to insolubilized histamine, the elimination of which restores the level of response to almost normal.
利用一种基于单核细胞对固定化组胺的差异亲和力的方法，我们能够区分健康对照受试者和结节病患者之间T细胞亚群组成的若干差异。在10例结节病患者中，有5例T细胞对三种有丝分裂原的反应能力严重降低，而其余5例则与正常受试者的反应相似。在去除粘附于组胺柱的外周血单核细胞（PBMC）亚群后，正常对照受试者未结合细胞对有丝分裂原的反应明显低于分离前获得的值。五例活动性结节病患者的组胺未结合淋巴细胞，其未分离的PBMC对有丝分裂原反应较差，分离后对有丝分裂原的反应明显优于分离前。这种反应模式以前仅在自身免疫性疾病患者的PBMC中出现。似乎一些结节病患者拥有具有抑制活性且对固定化组胺有特异性亲和力的T淋巴细胞亚群，去除该亚群可使反应水平恢复到几乎正常。
