Warzecha Joanna, Oczkowicz Maria, Rubis Dominika, Fornal Agnieszka, Szmatoła Tomasz, Bugno-Poniewierska Monika
Department of Molecular Biology of Animals National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland.
University Centre of Veterinary Medicine, University of Agriculture in Kraków, 30-059 Kraków, Poland.
Animals (Basel). 2019 Sep 27;9(10):737. doi: 10.3390/ani9100737.
The aim of this study was to evaluate the genetic variability of the White Kołuda goose and 12 conservative flocks: Kielecka, Podkarpacka, Garbonosa, Pomerian, Rypinska, Landes, Lubelska, Suwalska, Kartuska, Romanska, Slowacka, and Kubanska, maintained in Poland using microsatellite data. The genetic diversity of geese kept in Poland remains poorly analyzed at the molecular level. In total 392 samples were examined with the usage of 15 microsatellite markers. 119 alleles were identified and the number of alleles per locus ranged from 1 to 13. The highest number of alleles was observed in TTUCG5 (16) and the lowest in CAUD-G007 (2), while CKW47 was monomorphic. The lowest value of expected heterozygosity (H) was observed in Landes, while the highest in Romanska. Similarly, the observed heterozygosity (H) was the lowest in Landes but the highest in Kartuska. The polymorphism information content (PIC) indicates loci TTUCG5 as the most valuable microsatellite marker among those examined. The Structure software was used for the first time to identify goose populations, revealing high admixture between breeds and their close genetic propinquity. Moreover, the presented panel of microsatellite markers remained polymorphic and is useful for population studies of geese and assessment of genetic diversity.
本研究旨在利用微卫星数据评估波兰养殖的白科武达鹅以及12个传统鹅群（基莱茨卡、波德卡尔帕卡、加尔博诺萨、波美拉尼亚、雷平斯卡、朗德、卢贝尔斯卡、苏瓦尔卡、卡尔图斯卡、罗曼斯卡、斯洛瓦茨卡和库班斯卡）的遗传变异性。波兰养殖鹅的遗传多样性在分子水平上仍未得到充分分析。总共使用15个微卫星标记对392个样本进行了检测。共鉴定出119个等位基因，每个位点的等位基因数量从1到13不等。在TTUCG5位点观察到的等位基因数量最多（16个），在CAUD - G007位点最少（2个），而CKW47位点呈单态性。预期杂合度（H）在朗德鹅群中最低，在罗曼斯卡鹅群中最高。同样，观察到的杂合度（H）在朗德鹅群中最低，但在卡尔图斯卡鹅群中最高。多态信息含量（PIC）表明，在所检测的微卫星标记中，TTUCG5位点是最有价值的。首次使用Structure软件来识别鹅群，结果显示各品种之间存在高度混合以及它们在遗传上的紧密亲缘关系。此外，所展示的微卫星标记组合仍然具有多态性，可用于鹅的群体研究和遗传多样性评估。
