Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
PLoS One. 2019 Oct 3;14(10):e0221774. doi: 10.1371/journal.pone.0221774. eCollection 2019.
The general population is exposed to bisphenol A (BPA) orally, parenterally, transdermally, and environmentally as a result of the use of BPA in food packaging, plastics, and personal care products. The majority of the population nowadays (91-99%) has detectable levels of BPA inside their body. In this study, we successfully performed an inexpensive, rapid, and simple protein precipitation procedure for extraction of BPA from human plasma, followed by analysis by LC-MS/MS. This method was specifically developed for handling large numbers of samples with minimum cost and volume of sample. The developed method was accurate, precise, and reproducible for quantification of BPA from human plasma samples in the concentration range of 10-2000 ng/mL. The method was performed on samples from 150 healthy volunteers who were enrolled in the study. The mean of observed BPA level was 2.22 ± 9.91 ng/mL. Higher BPA levels were observed for females compare to that of males (p-value = 0.002), the BPA levels were higher in participants 33 years of age and older compared to those less than 33 years of age (p-value = 0.000), then the BPA levels higher in subjects with tap water as source of drinking (p-value = 0.005). This method may be valuable for general risk assessment of BPA for a large and varied population because of its efficiency and economical aspects.
由于双酚 A（BPA）在食品包装、塑料和个人护理产品中的应用，普通人群通过口服、注射、经皮和环境途径接触到 BPA。现在，大多数人（91-99%）体内都能检测到 BPA。在这项研究中，我们成功地进行了一种经济、快速且简单的蛋白质沉淀程序，从人血浆中提取 BPA，然后通过 LC-MS/MS 进行分析。该方法是专门为处理大量样本而开发的，成本最低，样本量最小。该方法准确、精密且可重现，可用于在 10-2000ng/mL 的浓度范围内定量人血浆样品中的 BPA。该方法在 150 名参加研究的健康志愿者的样本上进行。观察到的 BPA 水平平均值为 2.22±9.91ng/mL。与男性相比，女性的 BPA 水平更高（p 值=0.002），33 岁及以上参与者的 BPA 水平高于 33 岁以下参与者（p 值=0.000），而以自来水为饮用水源的受试者的 BPA 水平更高（p 值=0.005）。由于该方法具有高效和经济的特点，因此对于大量不同人群的 BPA 一般风险评估可能具有重要价值。
