Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Level 4 Pathology, Cambridge Biomedical Campus, Cambridge, CB2 0QQ, UK.
Department of Paediatrics, University of Cambridge, Box 116, Cambridge Biomedical Campus, Cambridge, CB2 0QQ, UK.
Sci Rep. 2019 Oct 4;9(1):14321. doi: 10.1038/s41598-019-50693-0.
We tested the hypothesis that both postnatal feeding and conditions in utero affect lipid metabolism in infants. Infants who experienced restrictive growth conditions in utero and others exposed to maternal hyperglycaemia were compared to a control group with respect to feeding mode. Dried blood spots were collected from a pilot subset of infant participants of the Cambridge Baby Growth Study at 3mo. Groups: (a) a normal gestation (control, n = 40), (b) small for gestational age (SGA, n = 34) and (c) whose mothers developed hyperglycaemia (n = 59). These groups were further stratified by feeding mode; breastfed, formula-fed or received a mixed intake. Their phospholipid, glyceride and sterol fractions were profiled using direct infusion mass spectrometry. Statistical tests were used to identify molecular species that indicated differences in lipid metabolism. The abundance of several phospholipids identified by multivariate analysis, PC(34:1), PC(34:2) and PC-O(34:1), was 30-100% higher across all experimental groups. SM(39:1) was around half as abundant in in utero groups among breastfed infants only. The evidence from this pilot study shows that phospholipid metabolism is modulated by both conditions in utero and postnatal feeding in a cohort of 133 Caucasian infants, three months post partum.
我们检验了这样一个假设，即产后喂养和宫内条件都会影响婴儿的脂质代谢。我们比较了经历宫内限制生长条件和暴露于母亲高血糖的婴儿与对照组的喂养方式。在剑桥婴儿生长研究的婴儿参与者的试点子集中，收集了 3 个月大的干血斑。组：（a）正常妊娠（对照组，n=40），（b）小于胎龄儿（SGA，n=34）和（c）其母亲发生高血糖（n=59）。这些组还根据喂养方式进一步分层；母乳喂养、配方奶喂养或混合喂养。使用直接注入质谱法对其磷脂、甘油酯和甾醇部分进行了分析。统计检验用于识别指示脂质代谢差异的分子种类。通过多变量分析鉴定的几种磷脂的丰度，PC（34：1）、PC（34：2）和 PC-O（34：1），在所有实验组中高出 30-100%。只有母乳喂养的宫内组的 SM（39：1）丰度低一半左右。本试验研究的证据表明，磷脂代谢在 133 名高加索婴儿队列中，无论是宫内条件还是产后喂养，都受到调节，产后三个月。
