Isolation, Identification, and Synthesis of a New Prenylated Cinnamic Acid Derivative from Brazilian Green Propolis and Simultaneous Quantification of Bioactive Components by LC-MS/MS.

A new cinnamic acid derivative, ()-3-[4-hydroxy-3-(()-3-formyl-2-butenyl)phenyl]-2- propenoic acid () has been isolated from the ethanol extract of Brazilian green propolis along with three known cinnamic acid derivatives, 3,4-dihydroxy-5-prenyl-()-cinnamic acid (), capillartemisin A (), and 2,2-dimethylchromene-6-()-propenoic acid (), and a flavonoid, dihydrokaempferide () by liquid-liquid participation, a series of column chromatography and preparative HPLC. Their structures have been determined by spectroscopic analyses and chemical synthesis of compound . The simultaneous quantification of 20 constituents, including 10 cinnamic acid derivatives, 7 flavonoids, and 3 caffeoylquinic acid derivatives, has also been developed and validated using LC-MS/MS. The new compound was shown to activate PPAR α but not PPAR β or γ.