秋水仙碱和细胞松弛素对小鼠B细胞膜IgM和IgD脱落的不同作用。
Differential effects of colchicine and cytochalasins on the shedding of murine B cell membrane IgM and IgD.
作者信息
Emerson S G, Cone R E
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6582-6. doi: 10.1073/pnas.76.12.6582.
Lactoperoxidase (EC 1.11.1.7) catalyzed cell surface radioiodination was employed to monitor the fate of murine B cell membrane (mem) IgM and IgD on radiolabeled cells in short-term culture. Both mem-IgM and mem-IgD were shed from the cell surface with biphasic kinetics. The rapid phase of mem-IgD shedding was somewhat slower (half-time = 12 hr) than that of mem-IgM shedding (half-time = 7-8 hr). The effect of temperature, colchicine, and cytochalasin on the shedding of the two membrane immunoglobulin isotypes was determined. The shedding of mem-IgD was more energy dependent than that of mem-IgM and was sensitive to colchicine but not cytochalasin. Conversely, the shedding of mem-IgM was sensitive to cytochalasin but not colchicine. The results suggest that the mechanisms of shedding of mem-IgM and mem-IgD are qualitatively distinct and may be regulated by microfilaments or microtubules, respectively.
利用乳过氧化物酶（EC 1.11.1.7）催化的细胞表面放射性碘化来监测短期培养中放射性标记细胞上小鼠B细胞膜（mem）IgM和IgD的命运。膜IgM和膜IgD均以双相动力学从细胞表面脱落。膜IgD脱落的快速阶段比膜IgM脱落的快速阶段稍慢（半衰期 = 12小时）（膜IgM半衰期 = 7 - 8小时）。确定了温度、秋水仙碱和细胞松弛素对两种膜免疫球蛋白同种型脱落的影响。膜IgD的脱落比膜IgM的脱落更依赖能量，并且对秋水仙碱敏感，但对细胞松弛素不敏感。相反，膜IgM的脱落对细胞松弛素敏感，但对秋水仙碱不敏感。结果表明，膜IgM和膜IgD的脱落机制在性质上是不同的，可能分别由微丝或微管调节。
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