拟南芥中侧生器官边界结构域（LBD）基因家族的全基因组分析

Genome-Wide Analysis of the Lateral Organ Boundaries Domain (LBD) Gene Family in .

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.

出版信息

Int J Mol Sci. 2019 Oct 28;20(21):5360. doi: 10.3390/ijms20215360.

Lateral organ boundaries domain () proteins belong to a particular class of transcription factors of lateral organ boundary () specific domains that play essential roles in plant growth and development. However, a potato phylogenetic analysis of the family has not been fully studied by scholars and researchers. In this research, bioinformatics methods and the growth of potatoes were used to identify 43 proteins. We separated them into seven subfamilies: Ia, Ib, Ic, Id, Ie, IIa and IIb. The number of amino acids encoded by the potato LBD family ranged from 94 to 327. The theoretical isoelectric point distribution ranged from 4.16 to 9.12 Kda, and they were distributed among 10 chromosomes. The results of qRT-PCR showed that the expression levels of and were up-regulated under drought stress in the stem. The expression levels of and were down-regulated in leaves. We hypothesized that was down-regulated under drought stress, and that and up-regulation might help to maintain the normal metabolism of potato and enhance the potatoes' resistance to drought.

侧生器官边界域（LBD）蛋白属于侧生器官边界域（LBD）特异转录因子的一个特殊家族，在植物生长和发育中发挥着重要作用。然而，学者和研究人员尚未对马铃薯家族进行全面的系统发育分析。在这项研究中，我们使用生物信息学方法和马铃薯的生长情况，鉴定出 43 种蛋白质。我们将它们分为七个亚家族：Ia、Ib、Ic、Id、Ie、IIa 和 IIb。马铃薯 LBD 家族编码的氨基酸数范围为 94 到 327 个。理论等电点分布范围为 4.16 到 9.12 Kda，分布在 10 条染色体上。qRT-PCR 的结果表明，在茎中干旱胁迫下和的表达水平上调。在叶片中和的表达水平下调。我们假设在干旱胁迫下下调，而和的上调可能有助于维持马铃薯的正常代谢，并增强马铃薯对干旱的抗性。