Hasby Saad M, Safwat O, El-Guindy D, Raafat R, Elgendy D, Hasby E
Medical Parasitology, Tanta University, Faculty of Medicine, Tanta, Egypt.
Biochemistry, Tanta University, Faculty of Medicine, Tanta, Egypt.
Helminthologia. 2018 Oct 27;55(4):261-274. doi: 10.2478/helm-2018-0029. eCollection 2018 Dec.
Idiopathic Parkinson's (IP) is a neurodegenerative disease that is suspected to be due to exposure to infections during early life. Toxoplasmosishas been the only suspected parasitic infection in IP (Celik et al., 2010). Recently, some non-central nervous system bacterial and viral infections have been incriminated in IP (Çamcı & Oğuz, 2016). So in the current study, we tried to explore if the systemic inflammatory reactions triggered by some helminths like can induce Parkinsonian lesions in the brain, especially that the cerebral complications have been reported in 10-20% of infected patients . An experimental study was designed to assess the neurodegenerative and biomolecular changes that may occur in infected BALB/C mice in comparison to rotenone induced PD model and apparently healthy ones. The motor affection was significantly lesser in the infected mice than the Parkinson's model, but when the catalepsy score was calculated (through the grid and bar tests) it was found to be significantly higher in the infected mice than in the healthy ones. A significant increase in the blood advanced oxidative protein products (AOPP), IFN-γ, TGF-β, and brain DNA fragmentation was also detected in the infected mice. After histopathological examination, a significant increase in the cortical apoptotic neurons and Lewy's body were observed in the infected and the rotenone induced Parkinson's model sections. A significant decrease in the immunohistochemical expression of the tyrosine hydroxylase expression in the brain sections and the ELISA measured dopamine level in the brain homogenate was also reported in the infected mice group. This study findings may collectively suggest that the systemic inflammatory reactions and the oxidative stresses associated with some systemic helminthic infections like trichinellosis are possible to precipitate neurodegenerative lesions and biomolecular changes in the brain , and manifest with IPD later in life.
特发性帕金森病（IP）是一种神经退行性疾病，怀疑与早年接触感染有关。弓形虫病一直是IP中唯一被怀疑的寄生虫感染（Celik等人，2010年）。最近，一些非中枢神经系统细菌和病毒感染也被认为与IP有关（Çamcı和Oğuz，2016年）。因此，在当前的研究中，我们试图探讨某些蠕虫（如旋毛虫）引发的全身炎症反应是否会在大脑中诱发帕金森氏病变，特别是鉴于10%-20%的旋毛虫感染患者报告有脑部并发症。设计了一项实验研究，以评估与鱼藤酮诱导的帕金森病模型和明显健康的小鼠相比，旋毛虫感染的BALB/C小鼠可能发生的神经退行性和生物分子变化。感染旋毛虫的小鼠运动功能受影响程度明显低于帕金森病模型小鼠，但通过网格和杆状试验计算僵住症评分时，发现感染小鼠的僵住症评分明显高于健康小鼠。感染旋毛虫的小鼠血液中晚期氧化蛋白产物（AOPP）、干扰素-γ、转化生长因子-β以及脑DNA片段化也显著增加。组织病理学检查后，在感染旋毛虫的小鼠和鱼藤酮诱导的帕金森病模型切片中，观察到皮质凋亡神经元和路易小体显著增加。感染小鼠组脑切片中酪氨酸羟化酶表达的免疫组化表达以及脑匀浆中ELISA检测的多巴胺水平也显著降低。这项研究结果可能共同表明，与某些全身蠕虫感染（如旋毛虫病）相关的全身炎症反应和氧化应激可能会促使大脑发生神经退行性病变和生物分子变化，并在生命后期表现为特发性帕金森病。
