基于带不同正电荷金纳米颗粒的生物传感器：一种用于食源性病原体检测的无标记且灵敏的工具。

Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection.

作者信息

Bu Tong, Jia Pei, Liu Jinghan, Liu Yingnan, Sun Xinyu, Zhang Meng, Tian Yongming, Zhang Daohong, Wang Jianlong, Wang Li

机构信息

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.

出版信息

Food Chem X. 2019 Aug 23;3:100052. doi: 10.1016/j.fochx.2019.100052. eCollection 2019 Sep 30.

Gold nanoparticles (AuNPs)-based lateral flow strip (LFS) enables a quick screening of foodborne bacteria in point-of-care (POC) diagnostics but suffers from the cumbersome labeling of antibodies and poor sensitive performance. Here, we innovated a label-free immunoassay to detect pathogenic bacteria by introducing diversely positive charges functionalized AuNPs ((+) AuNPs). The (+) AuNPs can be loaded on negatively charged bacteria via electrostatic interaction, leading to a color labeling to target bacteria. Afterward, the (+) AuNPs-bacteria complex can be specifically captured by monoclonal antibody (McAb) immobilized on test (T)-line. Under optimum conditions, the proposed LFS exhibited a lowest detectable concentration of 10 CFU/mL for () and could be well applied in drinking water, lettuce and pork samples. Moreover, this novel (+) AuNPs-LFS possessed a universal applicability, which could also be used for detecting O157 ( O157) with a superior sensitivity (10 CFU/mL) and specificity.

基于金纳米颗粒（AuNPs）的侧向流动试纸条（LFS）能够在即时检测（POC）诊断中快速筛查食源细菌，但存在抗体标记繁琐和灵敏性能不佳的问题。在此，我们通过引入不同正电荷功能化的AuNPs（(+) AuNPs）创新了一种无标记免疫分析法来检测病原菌。(+) AuNPs可通过静电相互作用负载在带负电荷的细菌上，从而对目标细菌进行颜色标记。之后，(+) AuNPs-细菌复合物可被固定在检测（T）线上的单克隆抗体（McAb）特异性捕获。在最佳条件下，所提出的LFS对()的最低可检测浓度为10 CFU/mL，并且可很好地应用于饮用水、生菜和猪肉样品中。此外，这种新型的(+) AuNPs-LFS具有普遍适用性，还可用于检测O157（O157），具有优异的灵敏度（10 CFU/mL）和特异性。