Ma Da-Won, Lee Myoung-Ro, Hong Sung-Hee, Cho Shin-Hyeong, Lee Sang-Eun
Division of Vectors and Parasitic Diseases, Centers for Disease Control and Prevention, Osong 28159, Korea.
Korean J Parasitol. 2019 Oct;57(5):531-536. doi: 10.3347/kjp.2019.57.5.531. Epub 2019 Oct 31.
Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013-2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.
微小隐孢子虫和十二指肠贾第鞭毛虫是引起腹泻的主要寄生性病原体；然而，它们在韩国的流行情况尚不清楚。在此，我们进行了一项调查，以确定2013 - 2016年期间在韩国17个地区卫生环境研究所住院的8571例患者中，这两种引起急性腹泻的病原体的流行情况和基因型分布。通过巢式PCR检测微小隐孢子虫和十二指肠贾第鞭毛虫并进行基因分型，分别通过对糖蛋白60（Gp60）和β - 贾第虫蛋白基因进行测序对分离株进行分子特征分析。微小隐孢子虫和十二指肠贾第鞭毛虫的总体流行率分别为0.37%（n = 32）和0.55%（n = 47），两种病原体在9岁以下儿童中更为普遍。分子流行病学分析表明，微小隐孢子虫分离株属于IIa家族，亚型为IIaA13G2R1、IIaA14G2R1、IIaA15G2R1和IIaA18G3R1。对十二指肠贾第鞭毛虫β - 贾第虫蛋白基因片段的分析表明，所有阳性菌株均属于A群。这是韩国首次对如此大量腹泻患者中的微小隐孢子虫和十二指肠贾第鞭毛虫进行分子流行病学和亚型分析的报告。这些结果凸显了对这些人畜共患病原体进行持续监测的必要性，并为实施控制和预防策略提供了依据。此外，这些结果可能有助于对疫情爆发源进行流行病学调查。
