A POTENTIAL ZOONOTIC PARASITE: TRANSMISSION IN RATS, PIGS AND HUMANS IN WEST LOMBOK, INDONESIA.

BACKGROUND

is a neglected zoonotic disease, but with the expansion of the human community into the animal environment, its incidence is increasing. Animals such as rats and pigs can act as intermediate hosts and transmit to humans due to their proximity. Transmission occurs due to the ability of to survive in any new host. The research aimed to identify and describe the transmission of from animals to humans.

MATERIALS AND METHODS

This research was a cross sectional study and samples were collected from 84 rats caught in residential areas, 205 pigs, and 438 humans in West Lombok. Fecal samples were examined using polymerase chain reaction (PCR) and sequencing to isolate the presence of , and identify the genetic similarity of the parasites found in rats and pigs with those that infect humans.

RESULTS

The PCR results found in 4.76% (4/84) in rats; 6.34% 13/205) in pigs; and 0.91% (4/438) in humans. The sequencing results showed genetic kinship of in rats, pigs, and humans. Based on sequence confirmation from Gene Banks and edited using ClustalW with MEGA X software, there are genetic similarities between isolates from West Lombok and isolates of cattle from Uganda and isolates of pigs from Slovakia.

CONCLUSION

There are genetic similarities of in animals and humans, requiring that the Public Health programs in those contaminated areas must receive priority attention to prevent further transmission of these potentially fatal parasites.