Qu Yongsheng, Wang Liang, Guo Wei
Shandong Academy of Chinese Medicine, 7 Yanzishanxi Street, Jinan 250014, China.
J Anal Methods Chem. 2019 Oct 16;2019:8796276. doi: 10.1155/2019/8796276. eCollection 2019.
The leaves of (LMA) are crucial traditional Chinese medicine (TCM) of clearing heat. In ancient Chinese materia medica and the current Pharmacopoeia of the People's Republic of China, LMA are recorded to be harvested after frost for medicinal purpose. However, the reason and mechanism of this traditional usage have been still unknown so far. In this work, it was confirmed firstly that the antipyretic effect of LMA after frost was better than that of before frost significantly on feverish rats. Subsequently, the chemical profiles of LMA before and after frost were characterized by fingerprint, respectively. Then, the endemic peaks after frost and positive differential peaks were screened as the research object of spectrum-effect correlation by orthogonal signal correction partial least square discrimination (OPLS). Finally, a multivariable and continuous-index spectrum-effect correlation model coupled with OPLS was established. As a result, the antipyretic components of postfrost LMA were screened and identified as citric acid derivative and tryptophan which may be the synergistic material basis. The study can provide a scientific foundation for the enhancement of effects in the postfrost LMA. Moreover, the strategy of this research could provide a valuable reference for revealing the material basis of synergetic or antagonistic effects among other complex drug systems.
某植物（文中未明确写出具体植物名称，暂用LMA指代）的叶子是重要的清热类传统中药。在中国古代本草及现行的《中华人民共和国药典》中，记载LMA需在霜降后采收入药。然而，这种传统用法的原因及机制至今仍不明确。在本研究中，首先证实了霜降后LMA对发热大鼠的解热作用明显优于霜降前。随后，分别采用指纹图谱对霜降前后LMA的化学特征进行表征。然后，将霜降后的特有峰和正向差异峰作为正交信号校正偏最小二乘判别分析（OPLS）的谱效关系研究对象。最后，建立了基于OPLS的多变量连续指标谱效关系模型。结果筛选并鉴定出霜降后LMA的解热成分是柠檬酸衍生物和色氨酸，它们可能是协同作用的物质基础。该研究可为提高霜降后LMA的药效提供科学依据。此外该研究策略可为揭示其他复杂药物体系中协同或拮抗效应的物质基础提供有价值的参考。
