Noh Hyun-Ju, Park Yerin, Hong Soon Gyu, Lee Yung Mi
Division of Life Sciences, Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon 21990, Korea.
School of Earth and Environmental Sciences, Seoul National University, Gwanak-gu, Seoul 08826, Korea.
Microorganisms. 2021 Mar 15;9(3):607. doi: 10.3390/microorganisms9030607.
The diversity of lichen-associated bacteria from lichen taxa , , , , , and was investigated by sequencing of 16S gene amplicons. Physiological characteristics of the cultured bacterial isolates were investigated to understand possible roles in the lichen ecosystem. Proteobacteria (with a relative abundance of 69.7-96.7%) were mostly represented by the order Rhodospirillales. The 117 retrieved isolates were grouped into 35 phylotypes of the phyla Actinobacteria (27), Bacteroidetes (6), Deinococcus-Thermus (1), and Proteobacteria (Alphaproteobacteria (53), Betaproteobacteria (18), and Gammaproteobacteria (12)). Hydrolysis of macromolecules such as skim milk, polymer, and (hypo)xanthine, solubilization of inorganic phosphate, production of phytohormone indole-3-acetic acid, and fixation of atmospheric nitrogen were observed in different taxa. The potential phototrophy of the strains of the genus which were cultivated from a lichen for the first time was revealed by the presence of genes involved in photosynthesis. Altogether, the physiological characteristics of diverse bacterial taxa from Antarctic lichens are considered to imply significant roles of lichen-associated bacteria to allow lichens to be tolerant or competitive in the harsh Antarctic environment.
通过对16S基因扩增子进行测序，研究了地衣类群、、、、、和中与地衣相关的细菌的多样性。对培养的细菌分离株的生理特性进行了研究，以了解其在地衣生态系统中可能发挥的作用。变形菌门（相对丰度为69.7 - 96.7%）主要由红螺菌目代表。回收的117个分离株被分为放线菌门（27个）、拟杆菌门（6个）、嗜热栖热菌门（1个）和变形菌门（α-变形菌纲（53个）、β-变形菌纲（18个）和γ-变形菌纲（12个））的35个系统发育型。在不同类群中观察到了对大分子如脱脂乳、聚合物和（次）黄嘌呤的水解、无机磷酸盐的溶解、植物激素吲哚 - 3 - 乙酸的产生以及大气氮的固定。首次从地衣中培养出的属菌株的潜在光合营养通过参与光合作用的基因的存在得以揭示。总之，南极地衣中不同细菌类群的生理特性被认为意味着与地衣相关的细菌在地衣耐受或在恶劣的南极环境中竞争方面发挥着重要作用。
