Laboratory of Proteins Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology of Tunis (INSAT), The University of Tunis Carthage, Via Elio Chianesi 53, 00144, Tunis, Tunisia.
Department of Research, Diagnosis and Innovative Technologies, IRCCS-Regina Elena National Cancer Institute, UOSD SAFU, Via Elio Chianesi 53, 00144, Rome, Italy.
Mol Biol Rep. 2020 Feb;47(2):1283-1292. doi: 10.1007/s11033-019-05228-1. Epub 2019 Dec 20.
Hsa-mir-143 and hsa-let-7c have been reported to be deregulated in multiple neoplasms. The main purpose of this study was to investigate the expression of these miRNAs in bladder cancer (BCa) and to analyze the association between their expression profiles and clinical and epidemiological parameters. Ninety BCa specimens were included. Expression patterns of miR-143 and let-7c were assessed by qRT-PCR using Taqman specific probes. Validated and predicted targets of these miRNA's were identified using CSmiRTar and DAVID tools, respectively. miR-143 was downregulated in tumors compared to controls (mean fold-change (FC) = 0.076). Its expression was significantly higher in MIBC compared to NMIBC (p = 0,001). Its value as a potential biomarker discriminating non invasive tumors from the invasive ones was confirmed by ROC curve (AUC = 0.768; p = 0.0001). Also, this down-regulation positively correlates with frequency of tobacco use (p = 0,04) and chronic alcohol consumption (p = 0,04). Let-7c was overexpressed in BCa samples (mean (FC = 9.92) compared to non tumoral ones but was not associated to clinical and epidemiological parameters. A comprehensive overview of miR-143 targets and pathways implicated in BCa initiation, diagnosis or prognosis using bioinformatical analysis, was conducted. While both deregulated miRNAs may contribute to urothelial tumorigenesis, the deregulation of miR-143 was significantly correlated to epidemiological and clinical parameters.
hsa-mir-143 和 hsa-let-7c 在多种肿瘤中被报道存在失调。本研究的主要目的是研究这些 miRNA 在膀胱癌 (BCa) 中的表达情况，并分析其表达谱与临床和流行病学参数之间的关联。本研究纳入了 90 例 BCa 标本。采用 Taqman 特异性探针的 qRT-PCR 评估 miR-143 和 let-7c 的表达模式。分别使用 CSmiRTar 和 DAVID 工具验证和预测这些 miRNA 的靶标。与对照组相比，肿瘤中 miR-143 的表达下调（平均倍数变化 (FC) = 0.076）。其在 MIBC 中的表达显著高于 NMIBC（p = 0.001）。ROC 曲线证实其作为潜在的生物标志物区分非浸润性肿瘤与浸润性肿瘤的价值（AUC = 0.768；p = 0.0001）。此外，这种下调与吸烟频率（p = 0.04）和慢性酒精消费（p = 0.04）呈正相关。Let-7c 在 BCa 样本中表达上调（与非肿瘤样本相比，平均（FC = 9.92），但与临床和流行病学参数无关。通过生物信息学分析，对 miR-143 靶标和参与 BCa 发生、诊断或预后的途径进行了全面概述。虽然这两种失调的 miRNA 可能都有助于尿路上皮肿瘤的发生，但 miR-143 的失调与流行病学和临床参数显著相关。
