Gupta Sulagna, Chen Wei Ning
Interdisciplinary Graduate School, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
Residues and Resource Reclamation Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 CleanTech Loop, CleanTech One, #06-08, Singapore 637141, Singapore.
ACS Omega. 2019 Dec 12;4(26):21675-21683. doi: 10.1021/acsomega.9b01925. eCollection 2019 Dec 24.
Extracts were extricated from raw okara and okara fermented with using a clean, green protocol; water was used as the extraction solvent and coupled with ultrasound assistance for enhanced extraction. In vitro anti-oxidant analyses for antioxidant potential and capacity, superoxide scavenging activity, and nitric oxide scavenging activity validated that fermented okara yielded superior bioactive performance compared to raw okara. Fermented okara extracts showed no toxicity to erythrocytes and successfully prevented induced haemolysis. After 48 h incubation at the highest tested concentration (100 mg/mL), fermented okara extracts could inhibit HepG2 cells by 48.47 ± 5.28%, which was significantly different from their effects on NIH 3T3 cells. Gas chromatography-mass spectrometry characterization of extracts validated amino acids to be the chief fraction responsible for the detected bioactivity of the fermented okara extract. The results derived in this study open up the possibility that biofermented okara extract may be a potential novel sustainable nutraceutical.
采用清洁、环保的方案从生豆渣和发酵豆渣中提取提取物；以水作为提取溶剂，并结合超声辅助以增强提取效果。通过体外抗氧化分析评估抗氧化潜力和能力、超氧化物清除活性和一氧化氮清除活性，结果证实发酵豆渣比生豆渣具有更优异的生物活性性能。发酵豆渣提取物对红细胞无毒性，并成功预防了诱导的溶血。在最高测试浓度（100 mg/mL）下孵育48小时后，发酵豆渣提取物可抑制HepG2细胞48.47±5.28%，这与它们对NIH 3T3细胞的作用有显著差异。提取物的气相色谱-质谱表征证实氨基酸是发酵豆渣提取物检测到的生物活性的主要成分。本研究得出的结果表明，生物发酵豆渣提取物有可能成为一种潜在的新型可持续营养保健品。
