Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China.
Talanta. 2020 Mar 1;209:120527. doi: 10.1016/j.talanta.2019.120527. Epub 2019 Oct 31.
Photoacoustic (PA) effect has been widely applied in many fields, e.g., physics, chemistry and biomedicine. Herein, a miniaturized PA device is developed by integrating laser source, photo chopper, PA cell, microphone, and laptop for point-of-care testing in bioassay. With glucose assay as model, a piece of paper strip preloading chitosan, starch-potassium iodide (KI) and glucose oxidase (GOD) as lab-on-paper is employed for loading sample prior to PA detection. In the presence of glucose, the product generated on the paper strip would give rise to a strong PA signal in the PA cell under the irradiation of frequency-modulated laser at 520 nm via laptop readout. With a sample volume of 20 μL, a detection limit of 0.03 mM is obtained for glucose assay, along with a linear range of 0.08-1 mM. The accuracy and practicability of the present PA device is well demonstrated by detecting glucose in whole blood. Differing from the conventional PA instrument, the present PA device is really small in bulk with competitive sensitivity and excellent stability, offering a promising tool for point-of-care testing in bioassay.
光声（PA）效应已广泛应用于物理、化学和生物医学等多个领域。在此，我们开发了一种微型 PA 装置，该装置集成了激光源、光斩波器、PA 池、麦克风和笔记本电脑，可用于生物测定中的即时检测。以葡萄糖测定为例，使用一条预先加载壳聚糖、淀粉-碘化钾（KI）和葡萄糖氧化酶（GOD）的纸条作为纸上实验室，用于在 PA 检测之前加载样品。在存在葡萄糖的情况下，在 520nm 频率调制激光的照射下，纸条上生成的产物会在 PA 池内产生强烈的 PA 信号，通过笔记本电脑读取。对于葡萄糖测定，在 20μL 的样品体积下，检测限为 0.03mM，线性范围为 0.08-1mM。通过检测全血中的葡萄糖，证明了本 PA 装置具有良好的准确性和实用性。与传统的 PA 仪器不同，本 PA 装置体积小巧，具有竞争力的灵敏度和出色的稳定性，为生物测定中的即时检测提供了一种有前途的工具。
