Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.
Bam University of Medical Sciences, Bam, Iran.
Anal Bioanal Chem. 2020 Feb;412(4):1011-1024. doi: 10.1007/s00216-019-02326-8. Epub 2020 Jan 2.
Cabbage flower-like Ho/NiO nanostructure (CFL-Ho/NiO NSs) with significant electrocatalytic oxidation has been published for the first time. First, structure and morphology of CFL-Ho/NiO-NSs have been described by XRD, SEM, and EDX methods. Then, CFL-Ho/NiO-NSs have been applied as a modifier for simultaneous electrochemical detection of methotrexate (MTX) and carbamazepine (CBZ). Functions of the modified electrode have been dealt with through electrochemical impedance spectroscopy (EIS). It has been demonstrated that the electrode response has been linear from 0.001-310.0 μM with a limit of detection of 5.2 nM and 4.5 nM (3 s/m) through DPV for MTX and CBZ. Diffusion coefficient (D) and heterogeneous rate constant (k) have been detected for MTX and CBZ oxidation at the surface of the modified electrode. Moreover, CFL-Ho/NiO-NS/GCE has been employed for determining MTX and CBZ in urine and drug specimens. Outputs showed the analyte acceptable recovery. Therefore, the electrode could be applied to analyze both analytes in drug prescription and clinical laboratories. Graphical abstract Electrochemical sensor based on bifunctional cabbage flower-like Ho/NiO nanostructures modified glassy carbon electrode for simultaneous detecting methotrexate and carbamazepine was fabricated.
首次报道了具有显著电催化氧化性能的甘蓝花状 Ho/NiO 纳米结构（CFL-Ho/NiO NSs）。首先，通过 XRD、SEM 和 EDX 方法描述了 CFL-Ho/NiO-NSs 的结构和形态。然后，将 CFL-Ho/NiO-NSs 用作同时电化学检测甲氨蝶呤（MTX）和卡马西平（CBZ）的修饰剂。通过电化学阻抗谱（EIS）研究了修饰电极的功能。结果表明，通过 DPV，修饰电极对 MTX 和 CBZ 的线性响应范围分别为 0.001-310.0 μM，检测限分别为 5.2 nM 和 4.5 nM（3 s/m）。还检测了 MTX 和 CBZ 在修饰电极表面氧化的扩散系数（D）和非均相速率常数（k）。此外，CFL-Ho/NiO-NS/GCE 还用于测定尿样和药物样本中的 MTX 和 CBZ。结果表明，该分析物具有可接受的回收率。因此，该电极可用于药物处方和临床实验室中分析这两种分析物。 基于双功能甘蓝花状 Ho/NiO 纳米结构修饰玻碳电极的电化学传感器被用于同时检测甲氨蝶呤和卡马西平。
