Morris M E, Levy G
Department of Pharmaceutics, School of Pharmacy, State University of New York at Buffalo, Amherst 14260.
Anal Biochem. 1988 Jul;172(1):16-21. doi: 10.1016/0003-2697(88)90405-8.
An assay using nonsuppressed (single-column) anion chromatography was developed to determine the concentration of inorganic sulfate in biologic fluids. A conventional HPLC system with an anion-exchange column and conductimetric detector interfaced with an automatic injector and integrator was used. The mobile phase for the chromatography of urine and serum samples is 4 mM potassium hydrogen phthalate, pH 4.5, and potassium iodide is used as the internal standard. For cerebrospinal fluid samples, the mobile phase is modified by addition of 10% of a 4 mM phthalic acid solution. Results of the HPLC assay were found to correlate well (r = 0.991 and 0.999) with those of two commonly used spectrophotometric methods for urine and serum inorganic sulfate determinations. However, the concentrations determined by ion chromatography were 2.5 to 10% lower, possibly due to less assay interference by other substances following chromatographic separation of sulfate. Anion chromatography using a single-column system is a convenient and relatively inexpensive method with sufficient sensitivity for the determination of inorganic sulfate concentrations in urine, serum, and cerebrospinal fluid.
开发了一种使用非抑制（单柱）阴离子色谱法来测定生物体液中无机硫酸盐浓度的分析方法。使用了一个配备阴离子交换柱和电导检测器的传统高效液相色谱系统，该系统与自动进样器和积分仪相连。用于尿液和血清样品色谱分析的流动相是4 mM邻苯二甲酸氢钾，pH值为4.5，碘化钾用作内标。对于脑脊液样品，通过加入10%的4 mM邻苯二甲酸溶液来对流动相进行改性。高效液相色谱分析的结果与两种常用的分光光度法测定尿液和血清无机硫酸盐的结果具有良好的相关性（r = 0.991和0.999）。然而，离子色谱法测定的浓度低2.5%至10%，这可能是由于在硫酸盐色谱分离后其他物质对分析的干扰较少。使用单柱系统的阴离子色谱法是一种方便且相对便宜的方法，对测定尿液、血清和脑脊液中的无机硫酸盐浓度具有足够的灵敏度。
