Cole D E, Scriver C R
J Chromatogr. 1981 Oct 9;225(2):359-67. doi: 10.1016/s0378-4347(00)80284-4.
The application of controlled flow anion chromatography to the assay of inorganic sulfate in biological fluids is described. The sulfate anion is separated from other anions by ion-exchange chromatography and quantitated conductimetrically. Coefficient of variance is 3.4%, about half that for the barium precipitation assay. Interference from heparin in plasma samples and unknown sources in tissue extract analysis is avoided. Sulfate levels in plasma are not different from those measured in serum after protein precipitation. Normal levels for sulfate concentration in human plasma, cerebrospinal fluid and hepatic tissue extract are reported.
本文描述了控制流动阴离子色谱法在生物流体中无机硫酸盐测定中的应用。硫酸根阴离子通过离子交换色谱法与其他阴离子分离，并通过电导法进行定量。变异系数为3.4%，约为钡沉淀法的一半。避免了血浆样品中肝素的干扰以及组织提取物分析中未知来源的干扰。蛋白质沉淀后血浆中的硫酸盐水平与血清中测得的水平无差异。报告了人血浆、脑脊液和肝组织提取物中硫酸盐浓度的正常水平。
