Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
Division of Vascular Medicine, Department of Internal Medicine, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
Int J Mol Sci. 2020 Jan 2;21(1):314. doi: 10.3390/ijms21010314.
Oxidative stress plays a pivotal role in the pathogenesis of cardiovascular diseases (CVD). Postmenopausal women have an increased risk of developing CVD due to decreased estrogen availability, which is accompanied by increased oxidative stress. Serum free thiols (R-SH) provide a robust and powerful read-out of systemic oxidative stress. In this study, we aimed to establish serum levels of free thiols and explore associations between free thiols and demographic, clinical, and biochemical parameters related to obesity and the risk for developing CVD in both pre- and postmenopausal women. Serum free thiols were measured in a cohort consisting of healthy pre- ( = 223) and postmenopausal ( = 118) Omani women. Postmenopausal women had significantly lower levels of serum free thiols as compared to premenopausal women (762.9 ± 85.3 vs. 780 ± 80.9 μM, age-adjusted < 0.001). Women's age was positively associated with serum free thiol levels in premenopausal women (β = 0.36, = 0.002), whereas an inverse association was observed in postmenopausal women (β = -0.29, = 0.002). Homocysteine levels were significantly inversely associated with serum free thiol levels in both pre- (β = -0.19, = 0.005) and postmenopausal (β = -0.20, = 0.032) women, independent from known cardiovascular risk factors. In this study, we show that postmenopausal women are affected by increased systemic oxidative stress, which independently associates with homocysteine levels.
氧化应激在心血管疾病 (CVD) 的发病机制中起着关键作用。由于雌激素水平下降，绝经后妇女患 CVD 的风险增加，同时氧化应激增加。血清游离巯基 (R-SH) 为系统氧化应激提供了一个强大而有力的读数。在这项研究中，我们旨在确定游离巯基的血清水平，并探讨游离巯基与与肥胖相关的人口统计学、临床和生化参数以及绝经前和绝经后妇女患 CVD 的风险之间的关系。在一个由健康的绝经前（n=223）和绝经后（n=118）阿曼妇女组成的队列中测量了血清游离巯基。与绝经前妇女相比，绝经后妇女的血清游离巯基水平显著降低（762.9±85.3 与 780±80.9 μM，年龄调整后 <0.001）。在绝经前妇女中，女性年龄与血清游离巯基水平呈正相关（β=0.36， =0.002），而在绝经后妇女中则呈负相关（β=-0.29， =0.002）。同型半胱氨酸水平与绝经前（β=-0.19， =0.005）和绝经后（β=-0.20， =0.032）妇女的血清游离巯基水平呈显著负相关，独立于已知的心血管危险因素。在这项研究中，我们表明绝经后妇女受到全身性氧化应激增加的影响，这与同型半胱氨酸水平独立相关。
