Kagan R L, Schuette W H, Zierdt C H, MacLowry J D
J Clin Microbiol. 1977 Jan;5(1):51-7. doi: 10.1128/jcm.5.1.51-57.1977.
Lysis and filtration of blood culture specimens were combined with impedance detection of bacterial growth to facilitate the diagnosis of bacteremia. A blood lysis-filtration technique (Zierdt et al., 1976) was coupled to a simple, inexpensive automated detection system. The practical and technical aspects of the impedance detection system are discussed. This new blood culturing system was compared to a conventional system for 264 aerobic blood cultures. A 30-ml sample of the blood-broth mixture was withdrawn from the conventional aerobic blood culture bottle and processed in parallel. Excluding the isolation of the commonly recognized contaminants, the detection efficiency was 36% greater in the new system. A total of 53 blood cultures from 107 patients were positive by one or both methods. The new system detected 92% of the total number of positive cultures, compared with 56% detected by the routine method. The explanation of the differences is discussed.
血液培养标本的裂解和过滤与细菌生长的阻抗检测相结合，以促进菌血症的诊断。一种血液裂解过滤技术（齐尔德特等人，1976年）与一个简单、廉价的自动检测系统相结合。讨论了阻抗检测系统的实际和技术方面。将这种新的血液培养系统与传统系统进行了264次需氧血液培养的比较。从传统需氧血液培养瓶中抽取30毫升血-肉汤混合物样本，并进行平行处理。排除常见污染物的分离情况，新系统的检测效率比传统系统高36%。107名患者的总共53次血液培养通过一种或两种方法检测为阳性。新系统检测出阳性培养物总数的92%，而常规方法检测出的比例为56%。讨论了差异的原因。
