Zierdt C H
J Clin Microbiol. 1983 Apr;17(4):628-30. doi: 10.1128/jcm.17.4.628-630.1983.
A new blood lysis-filtration culture technique revealed a high incidence of Staphylococcus epidermidis in the blood of patients and of healthy people. Of 2,004 blood cultures from patients, the blood lysis method grew S. epidermidis in 233 (11.6%), whereas a conventional two-bottle culture system grew this organism in 48 (2.4%). To determine the incidence deriving from the skin, 100 mock blood cultures by each technique were performed. The antecubital fossa was prepared as for a phlebotomy. The needle was inserted through the skin but not into the vein. Needles were cultured by conventional and lysis-filtration culture. A total of 1 conventional culture of 100 (1%) and 2 lysis-filtration cultures of 100 (2%) grew S. epidermidis. Of 100 lysis-filtration and conventional control cultures with broth in place of blood, no cultures were positive. Blood samples from 8 of 117 (6.8%) healthy blood donors were positive for S. epidermidis by lysis-filtration, whereas no matching conventional cultures were positive. Phage typing patterns of skin and blood strains from selected individuals were the same. S. epidermidis isolates were often concomitant with isolates of bona fide pathogens. I conclude that intermittent, transient, asymptomatic S. epidermidis bacteremia occurs frequently in patients and in healthy humans.
一种新的血液溶解-过滤培养技术显示，患者和健康人的血液中表皮葡萄球菌的发生率很高。在来自患者的2004份血培养物中，血液溶解法培养出表皮葡萄球菌的有233份（11.6%），而传统的两瓶培养系统培养出该菌的有48份（2.4%）。为了确定源自皮肤的发生率，每种技术进行了100次模拟血培养。肘前窝按照静脉穿刺的方法进行准备。将针插入皮肤但不插入静脉。对针进行传统培养和溶解-过滤培养。100份传统培养中有1份（1%）、100份溶解-过滤培养中有2份（2%）培养出表皮葡萄球菌。在100份用肉汤代替血液的溶解-过滤和传统对照培养物中，没有培养物呈阳性。117名健康献血者中有8名（6.8%）的血液样本通过溶解-过滤法检测表皮葡萄球菌呈阳性，而相应的传统培养物均为阴性。选定个体的皮肤和血液菌株的噬菌体分型模式相同。表皮葡萄球菌分离株常与真正病原体的分离株同时出现。我的结论是，间歇性、短暂性、无症状的表皮葡萄球菌菌血症在患者和健康人中经常发生。
