He Zhijiang, Wu Jiaman, Xiao Bin, Xiao Shanqiu, Li Hongping, Wu Kaifeng
Shenzhen University General Hospital, Shenzhen, China.
Affiliated Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, China.
Front Pediatr. 2019 Dec 10;7:513. doi: 10.3389/fped.2019.00513. eCollection 2019.
The objective was to investigate the potential effect of gestational diabetes mellitus on the initial neonatal oral microbiome community structure. Oral samples were collected from 20 full-term, vaginally delivered newborns with sterile swabs. Nine of them had mothers diagnosed with gestational diabetes mellitus (GDM group), while 11 had non-diabetic mothers (NDM group). The oral microbiota was analyzed using multi-barcode 16S rRNA sequencing on Illumina MiSeq system. The results showed that the birth weight, gestational age and gestational weight gain were significantly higher in NDM group. There was a significant correlation between gestational age and birth weight. Neonatal oral microbiome was composed of five dominant phyla from , and . Compared to NDM group, a higher alpha diversity and reduction of phylum were observed in GDM group. Genus dominated in NDM group, while , and were overabundant in GDM group. Additionally, carbohydrate metabolism increased in NDM group, whereas amino acid metabolism, vitamin metabolism and lipopolysaccharide biosynthesis were more abundant in GDM group. This study showed a distinct oral microbiota profile in neonates born to mothers with GDM, which indicated that maternal diabetes status played an important role in neonatal initial oral microbiota.
目的是研究妊娠期糖尿病对新生儿初始口腔微生物群落结构的潜在影响。用无菌拭子从20名足月顺产的新生儿中采集口腔样本。其中9名新生儿的母亲被诊断为妊娠期糖尿病（GDM组），而11名新生儿的母亲无糖尿病（NDM组）。在Illumina MiSeq系统上使用多条形码16S rRNA测序分析口腔微生物群。结果显示，NDM组的出生体重、胎龄和孕期体重增加显著更高。胎龄与出生体重之间存在显著相关性。新生儿口腔微生物群由来自……的五个优势门组成。与NDM组相比，GDM组观察到更高的α多样性和……门的减少。NDM组中……属占主导，而GDM组中……、……和……属过度丰富。此外，NDM组中碳水化合物代谢增加，而GDM组中氨基酸代谢、维生素代谢和脂多糖生物合成更为丰富。本研究显示，患有GDM的母亲所生新生儿的口腔微生物群特征明显不同，这表明母亲的糖尿病状态在新生儿初始口腔微生物群中起重要作用。
