Institute for Nanoscience and Nanotechnology, Sharif University of Technology, P.O. Box 11365-11155, Tehran, Iran.
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110432. doi: 10.1016/j.msec.2019.110432. Epub 2019 Nov 15.
Coaxial electrospinning with the ability to use simultaneously two separate solvents provides a promising strategy for drug delivery. Nevertheless, controlled release of hydrophilic and sensitive therapeutics from slow biodegradable polymers is still challenging. To address this gap, we fabricated core-sheath fibers for dual delivery of lysozyme, as a model protein, and phenytoin sodium as a small therapeutic molecule. The sheath was processed by a gelatin solution while the core fibers were fabricated from an aqueous gelatin/PVA solution. Microstructural studies by transmission and scanning electron microscopy reveal the formation of homogeneous core-sheath nanofibers with an outer and inner diameter of 180 ± 48 nm and 106 ± 30 nm, respectively. Thermal gravimetric analysis determines that the mass loss of the core-sheath fibers fall between the mass loss values of individual sheath and core fibers. Swelling studies indicate higher water absorption of the core-sheath mat compared to the separate sheath and core membranes. In vitro drug release studies in Phosphate Buffered Saline (PBS) determine sustained release of the therapeutics from the core-sheath structure. The release trails three stages including non-Fickian diffusion at the early stage followed by the Fickian diffusion mechanism. The present study shows a useful approach to design core-sheath nanofibrous membranes with controlled and programmable drug release profiles.
同轴静电纺丝能够同时使用两种不同的溶剂，为药物输送提供了一种很有前途的策略。然而，从缓慢生物降解的聚合物中控制释放亲水性和敏感的治疗药物仍然具有挑战性。为了解决这一差距，我们制备了载溶菌酶（作为模型蛋白）和苯妥英钠（作为小分子治疗药物）的芯-壳纤维，用于双重递药。壳层由明胶溶液加工而成，而芯纤维则由明胶/PVA 水溶液制成。透射电子显微镜和扫描电子显微镜的微观结构研究表明，形成了均匀的核-壳纳米纤维，其外径和内径分别为 180±48nm 和 106±30nm。热重分析确定，芯-壳纤维的质量损失介于单独的壳层和芯层纤维的质量损失值之间。溶胀研究表明，与单独的壳层和芯层膜相比，芯-壳垫具有更高的吸水性。在磷酸盐缓冲盐水（PBS）中的体外药物释放研究表明，治疗药物从芯-壳结构中持续释放。释放试验分为三个阶段，包括早期的非菲克扩散，随后是菲克扩散机制。本研究展示了一种设计具有可控和可编程药物释放特性的芯-壳纳米纤维膜的有用方法。
