Khan Merajuddin, Alkhathlan Hamad Z, Khan Shams Tabrez
Department of Chemistry, College of Science, King Saud University, P.O.Box 2455, Riyadh 11451, Saudi Arabia.
Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 2002002 UP, India.
Pathogens. 2020 Jan 16;9(1):66. doi: 10.3390/pathogens9010066.
The use of organic components from plants as an alternative antimicrobial agent is becoming popular due to the development of drug-resistance in various pathogens. Essential oils from fresh (MF-1) and dried (MD-1) roots of L. were extracted and benzyl isothiocynate was determined as their chief constituent using GC-MS and GC-FID. The antibiofilm and antimicrobial activities of MD-1 and MF-1 against a dental caries causing bacteria were determined using multiple assays. These activities were compared with chlorhexidine digluconate (CHX) and clove oil, well known antimicrobial agents for oral hygiene. Essential oils demonstrated IC values (10-11 µg/mL) comparable to that of CHX, showed a significant reduction (82 ± 7-87 ± 6%) of the biofilm formation at a very low concentration. These results were supported by RT-PCR studies showing change in the expression levels of E, B, A and A genes involved in autolysis, biofilm formation and oxidative stress, respectively. The results presented in this study show the robust bactericidal and antibiofilm activity of MD-1 and MF-1 against which is comparable to Chlorhexidine digluconate. Our results suggest that these essential oils can be as effective as CHX and hence can serve as a good alternative antimicrobial agent for oral hygiene.
由于各种病原体中耐药性的发展，使用植物中的有机成分作为替代抗菌剂正变得越来越流行。从L.的新鲜（MF - 1）和干燥（MD - 1）根中提取了精油，并使用气相色谱 - 质谱联用仪（GC - MS）和气相色谱 - 氢火焰离子化检测器（GC - FID）确定苄基异硫氰酸酯为其主要成分。使用多种测定方法确定了MD - 1和MF - 1对一种致龋细菌的抗生物膜和抗菌活性。将这些活性与洗必泰葡萄糖酸盐（CHX）和丁香油（口腔卫生中众所周知的抗菌剂）进行了比较。精油显示出与CHX相当的IC值（10 - 11μg/mL），在非常低的浓度下生物膜形成显著减少（82±7 - 87±6%）。逆转录 - 聚合酶链反应（RT - PCR）研究支持了这些结果，该研究分别显示了参与自溶、生物膜形成和氧化应激的E、B、A和A基因表达水平的变化。本研究中呈现的结果表明MD - 1和MF - 1对具有强大的杀菌和抗生物膜活性，这与洗必泰葡萄糖酸盐相当。我们的结果表明，这些精油可以与CHX一样有效，因此可以作为口腔卫生的良好替代抗菌剂。
