Jiang Yueping, Zi Wen, Pei Zhifang, Liu Shao
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China.
Institute of Hospital Pharmacy, Central South University, Changsha 410008, China.
Saudi Pharm J. 2018 Jul;26(5):656-664. doi: 10.1016/j.jsps.2018.02.026. Epub 2018 Feb 14.
Two novel polysaccharides, polysaccharide I (LNP I) and polysaccharide II (LNP II), were extracted and purified from , and a sulfated polysaccharide, polysaccharide III (LNP III), with a substitution degree of 0.62 was prepared from LNPI. The structures of the LNPs were preliminarily characterized using high performance size exclusion chromatography (HPSEC), gas chromatography-mass spectrometry (GC-MS), Fourier transformed infrared spectrometry (FT-IR), and nuclear magnetic resonance (NMR) spectrometry. In addition, evaluation of the antioxidant activity of the LNPs showed that they could significantly increase the proliferation of RAW264.7 macrophages ( < 0.05) and improve the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) based on cell model of HOinduced oxidative damage. This suggested that these LNPs may be used as potential antioxidants.
从[具体来源未给出]中提取并纯化出两种新型多糖，即多糖I（LNP I）和多糖II（LNP II），并由LNP I制备出取代度为0.62的硫酸化多糖多糖III（LNP III）。使用高效尺寸排阻色谱法（HPSEC）、气相色谱 - 质谱联用仪（GC - MS）、傅里叶变换红外光谱仪（FT - IR）和核磁共振（NMR）光谱仪对LNP的结构进行了初步表征。此外，对LNP抗氧化活性的评估表明，基于HO诱导的氧化损伤细胞模型，它们可显著提高RAW264.7巨噬细胞的增殖能力（ < 0.05），并提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH - Px）的活性。这表明这些LNP可能用作潜在的抗氧化剂。
