Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
Food Chem Toxicol. 2020 Mar;137:111139. doi: 10.1016/j.fct.2020.111139. Epub 2020 Jan 23.
We investigated the protective effect and mechanism of selenium-enriched yeast (SY) on caecal injury induced by ochratoxin A (OTA) in broilers. Eighty broiler chickens of 1-day-old with similar weight were randomly assigned to Control group, OTA group, SY group and OTA + SY group, and were intragastricaly administered with OTA and SY for 21 consecutive days. The results showed that SY could reduce the caecal pathological injuries and could inhibit oxidative stress caused by OTA exposure. The OTA + SY group showed a statistically significant (p < 0.01) reduction in the level of MDA, IL-1β, IL-6 and IFN-γ, whereas the levels of GSH, SOD activity and IL-10 were significantly increased (p < 0.01). By regulating TLR4/MYD88 signaling pathway, SY inhibited the expression of NF-κB, increased the expression of tight junction-related genes Claudin-1, Occludin and ZO-1, and antagonized the intestinal barrier injury caused by OTA exposure. Moreover, the microbial diversity analyses indicated that SY could intervene changes in the diversity of gut microbiota and the imbalance of gut microbiota caused by OTA. SY could relieve caecal pathological injuries, alleviate OTA-induced caecal oxidative stress and inflammatory response, increase the gut microbial diversity and protect broiler's intestinal barrier from injury.
我们研究了富硒酵母（SY）对鸡盲肠损伤的保护作用及其机制，该损伤由赭曲霉毒素 A（OTA）引起。我们将 80 只 1 日龄、体重相似的肉鸡随机分为对照组、OTA 组、SY 组和 OTA+SY 组，连续 21 天经口给予 OTA 和 SY。结果表明，SY 可减轻盲肠的病理损伤，抑制 OTA 暴露引起的氧化应激。与 OTA+SY 组相比，MDA、IL-1β、IL-6 和 IFN-γ 水平显著降低（p<0.01），而 GSH、SOD 活性和 IL-10 水平显著升高（p<0.01）。SY 通过调节 TLR4/MYD88 信号通路，抑制 NF-κB 的表达，增加紧密连接相关基因 Claudin-1、Occludin 和 ZO-1 的表达，拮抗 OTA 暴露引起的肠道屏障损伤。此外，微生物多样性分析表明，SY 可以干预肠道微生物多样性的变化和 OTA 引起的肠道微生物失衡。SY 可减轻盲肠的病理损伤，缓解 OTA 诱导的盲肠氧化应激和炎症反应，增加肠道微生物多样性，保护肉鸡肠道屏障免受损伤。
