School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
State Key Laboratory of Applied Organic Chemistry & College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
Appl Biochem Biotechnol. 2024 Aug;196(8):5064-5079. doi: 10.1007/s12010-023-04785-3. Epub 2023 Nov 28.
In this paper, four novel hydrazine fluorescent probes X-X with bis-chalcone structure were designed and synthesized. Through the measurement of its optical properties, it is found that it can quickly identify hydrazine, high sensitivity, low detection limit, and good anti-interference ability. The recognition of hydrazine by probes X-X is not affected in the pH range of 4-10, X has the highest sensitivity, and the detection limit is as low as 0.336 × 10 M. Through Gaussian quantization calculation of probe molecules and their reaction products with hydrazine, it is speculated that the recognition mechanism is the closure of intramolecular charge transfer effect. In addition, the cytotoxicity and imaging of HeLa cells were tested, which showed that probes X-X could be used to detect hydrazine in cells.
在本文中，设计并合成了四个具有双查尔酮结构的新型肼荧光探针 X-X。通过对其光学性质的测量，发现它可以快速识别肼，具有高灵敏度、低检测限和良好的抗干扰能力。探针 X-X 对肼的识别不受 pH 值在 4-10 范围内的影响，X 具有最高的灵敏度，检测限低至 0.336×10-6M。通过对探针分子及其与肼反应产物的高斯量化计算，推测其识别机制是分子内电荷转移效应的关闭。此外，还测试了探针 X-X 对 HeLa 细胞的细胞毒性和成像，表明探针 X-X 可用于检测细胞中的肼。
