Department of Chemical Engineering, Kermanshah University of Technology, Kermanshah, Iran.
Department of Chemistry, Faculty of Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
Talanta. 2020 Apr 1;210:120641. doi: 10.1016/j.talanta.2019.120641. Epub 2019 Dec 10.
The early diagnosis of cancer increases the chance of its treatment. In this study, an enzyme-free sandwich type of immunosensors is presented for prostate specific antigen (PSA), as prostate cancer biomarker. In this regard, the quantum dots (QDs) and magnetic metal-organic frameworks (MOFs) were used for labeling the secondary anybody and modifying the electrode surface, respectively. Herein, a bare glassy carbon electrodes is modified with iron magnetic nanoparticles with a core shell MOFs (magnetic framework Fe3O4@ TMU-10) and the primary antibodies (Ab) are immobilized on the modified electrode. Moreover, the second antibody is successfully conjugated to a nickel-cadmium quantum dots as an electroactive non-enzymatic probe. The prepared immunosensor shows a linear range between 1 pg mL- 100 ng mL and the detection limit of 0.45 pg mL with acceptable selectivity, reproducibility and stability. The proposed sensor is also applied for determination of PSA in human serum samples and the results is confirmed by a reference method, which indicates its ability for clinical monitoring of the tumor marker.
癌症的早期诊断增加了治疗的机会。在这项研究中，提出了一种无酶三明治型免疫传感器，用于检测前列腺特异性抗原（PSA）作为前列腺癌生物标志物。在这方面，量子点（QDs）和磁性金属有机骨架（MOFs）分别用于标记二级抗体和修饰电极表面。在此，将带有核壳 MOFs（磁性框架 Fe3O4@TMU-10）的铁磁性纳米粒子修饰在玻碳电极上，并将一级抗体（Ab）固定在修饰的电极上。此外，将第二抗体成功地共轭到镍-镉量子点上，作为电活性非酶探针。制备的免疫传感器在 1 pg mL-100 ng mL 的线性范围内显示出线性关系，检测限为 0.45 pg mL，具有可接受的选择性、重现性和稳定性。该传感器还用于测定人血清样品中的 PSA，并用参考方法进行了验证，表明其具有用于肿瘤标志物临床监测的能力。
