Cai Zhiliang, Zhang Qiaoxuan, Xia Ziqiang, Zheng Songbai, Zeng Lilan, Han Liqiao, Yan Jun, Ke Peifeng, Zhuang Junhua, Wu Xinzhong, Huang Xianzhang
1Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
2Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510120 China.
Nutr Metab (Lond). 2020 Jan 17;17:8. doi: 10.1186/s12986-020-0427-7. eCollection 2020.
We aimed to describe the 25-hydroxyvitamin D (25(OH)D) status of southern Chinese individuals by a high-accuracy liquid chromatography tandem mass spectrometry (LC-MS/MS) method which can trace to reference measurement procedure.
From January 2018 to June 2019, a total of 4775 southern Chinese individuals were evaluated in our study. The serum levels of parathyroid hormone (PTH) were detected simultaneously in 162 cases. 25(OH)D was determined by LC-MS/MS, and PTH was detected using routine automated analysers. The distribution of the concentration, prevalence and seasonal variability of 25(OH)D in males and females of different age groups were studied.
The mean 25(OH)D concentration in our study was 32.57 ng/mL (4.20-101.40 ng/mL). The global 25(OH)D concentration in males was higher than that in females of different age group. The prevalence of vitamin D deficiency (< 20 ng/mL) in females (16.65%) was higher than that in males (6.83%). The prevalence of vitamin D deficiency (< 20 ng/mL) was most common in winter (22.98% of all women and 15.49% of all men). 25(OH)D concentrations were higher in those from whom blood samples were collected in summer and autumn than in winter and spring. 25(OH)D was detected in 672 serum samples (14.07%). In addition, there was a negative correlation between the concentrations of 25(OH)D and serum PTH ( = - 0.149, < 0.05).
Our study demonstrated that the average serum 25(OH)D concentration in southern Chinese individuals was higher than that in other Chinese cohorts by a high-accuracy LC-MS/MS method. The global 25(OH)D concentration in males was higher than that in females of different ages, and the prevalence of vitamin D deficiency in females was higher than that in males. Seasonal change was an important aspect of 25(OH)D concentration in young and middle-aged people but became less relevant for that in older subjects. 25(OH)D detection was of minor practical significance in our study. In addition, we also found that there was a negative correlation between the serum levels of 25(OH)D and PTH in southern Chinese individuals.
我们旨在通过一种可溯源至参考测量程序的高精度液相色谱串联质谱法（LC-MS/MS）描述中国南方人群的25-羟基维生素D（25(OH)D）状态。
2018年1月至2019年6月，共有4775名中国南方人群参与了我们的研究。同时对162例患者检测了甲状旁腺激素（PTH）的血清水平。采用LC-MS/MS测定25(OH)D，使用常规自动分析仪检测PTH。研究了不同年龄组男性和女性25(OH)D浓度的分布、患病率及季节变化。
本研究中25(OH)D的平均浓度为32.57 ng/mL（4.20 - 101.40 ng/mL）。不同年龄组男性的总体25(OH)D浓度高于女性。女性维生素D缺乏（<20 ng/mL）的患病率（16.65%）高于男性（6.83%）。维生素D缺乏（<20 ng/mL）的患病率在冬季最为常见（所有女性中的患病率为22.98%，所有男性中的患病率为15.49%）。夏季和秋季采集血样者的25(OH)D浓度高于冬季和春季。在672份血清样本中检测到了25(OH)D（14.07%）。此外，25(OH)D浓度与血清PTH之间存在负相关（r = -0.149，P < 0.05）。
我们的研究表明，通过高精度LC-MS/MS方法，中国南方人群的血清25(OH)D平均浓度高于其他中国人群队列。不同年龄男性的总体25(OH)D浓度高于女性，女性维生素D缺乏的患病率高于男性。季节变化是中青年人群25(OH)D浓度的一个重要方面，但对老年人群而言其相关性较小。在我们的研究中，25(OH)D检测的实际意义不大。此外，我们还发现中国南方人群血清25(OH)D水平与PTH之间存在负相关。
