Physiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), Barcelona 08028, Spain.
Nutrition and Food Safety Research Institute (INSA-UB), Santa Coloma de Gramenet 08921, Spain.
Nutrients. 2020 Jan 25;12(2):319. doi: 10.3390/nu12020319.
The present study aimed to analyze the rat breast milk profile of fatty acids (FA), immunoglobulins (Ig), microbiota, and their relationship, and to further assess their associations in the mother-offspring pair. Dams were monitored during the three weeks of gestation, allowed to deliver at term, and followed during two weeks of lactation. At the end of the study, milk was obtained from the dams for the analysis of fatty acids, microbiota composition, immunoglobulins, and cytokines. Moreover, the cecal content and plasma were obtained from both the dams and pups to study the cecal microbiota composition and the plasmatic levels of fatty acids, immunoglobulins, and cytokines. Rat breast milk lipid composition was ~65% saturated FA, ~15% monounsaturated FA, and ~20% polyunsaturated FA. Moreover, the proportions of IgM, IgG, and IgA were ~2%, ~88%, and ~10%, respectively. Breast milk was dominated by members of , and phyla. In addition, forty genera were shared between the milk and cecal content of dams and pups. The correlations performed between variables showed, for example, that all IgGs subtypes correlated between the three compartments, evidencing their association in the mother-milk-pup line. We established the profile of FA, Ig, and the microbiota composition of rat breast milk. Several correlations in these variables evidenced their association through the mother-milk-pup line. Therefore, it would be interesting to perform dietary interventions during pregnancy and/or lactation that influence the quality of breast milk and have an impact on the offspring.
本研究旨在分析大鼠母乳中脂肪酸 (FA)、免疫球蛋白 (Ig)、微生物群及其相互关系，并进一步评估它们在母婴对子中的关联。在妊娠的三周内监测母鼠，使其足月分娩，并在哺乳期的两周内进行跟踪。在研究结束时，从母鼠中获取母乳进行 FA、微生物群组成、免疫球蛋白和细胞因子分析。此外，从母鼠和幼崽中获取盲肠内容物和血浆，以研究盲肠微生物群组成以及血浆中 FA、免疫球蛋白和细胞因子的水平。大鼠母乳的脂质组成约为 65%饱和 FA、15%单不饱和 FA 和 20%多不饱和 FA。此外，IgM、IgG 和 IgA 的比例分别约为 2%、88%和 10%。母乳主要由 、 和 门的成员组成。此外，母鼠和幼崽的母乳和盲肠内容物中共有 40 个属。对变量进行的相关性分析表明，所有 IgG 亚型在三个隔室之间相互关联，证明它们在母-乳-幼线中存在关联。我们建立了大鼠母乳中 FA、Ig 和微生物群组成的特征。这些变量之间的相关性表明，它们通过母-乳-幼线相互关联。因此，在妊娠和/或哺乳期进行影响母乳质量并对后代产生影响的饮食干预将是有趣的。
